Anticorps Polyclonal de lapin anti-GTPBP9

• Phare
• Validé par KD/KO

GTPBP9 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (2)

N° de cat : 16371-1-AP

Synonymes

GTP binding protein 9, GTPBP9, Obg like ATPase 1, OLA1, PRO2455, PTD004

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Galerie de données de validation

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  WB analysis of Jurkat using 16371-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16371-1-AP (GTPBP9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16371-1-AP

  WB result of GTPBP9 antibody (16371-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GTPBP9 transfected HepG2 cells.

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  WB analysis of MCF-7 using 16371-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16371-1-AP (GTPBP9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16371-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16371-1-AP (GTPBP9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 16371-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16371-1-AP (GTPBP9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16371-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16371-1-AP (GTPBP9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16371-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16371-1-AP (GTPBP9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 16371-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16371-1-AP (GTPBP9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 16371-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 16371-1-AP (GTPBP9 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HepG2, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

16371-1-AP cible GTPBP9 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GTPBP9 Protéine recombinante Ag9283
• Nom complet: Obg-like ATPase 1
• Masse moléculaire calculée: 396aa,45 kDa; 278aa,31 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC013925
• Symbole du gène: OLA1
• Identification du gène (NCBI): 29789
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Clin Exp Pharmacol Physiol; OBG-like ATPase 1 inhibition attenuates angiotensin II-induced hypertrophic response in human ventricular myocytes via GSK-3beta/beta-catenin signalling.; Authors - Gayathri Narasimhan ; KD Validated
human,mouse	WB	medRxiv; Identification and development of Tetra-ARMS PCR-based screening test for a genetic variant of OLA1 (Tyr254Cys) in the human failing heart; Authors - Praveen K Dubey

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

S (Verified Customer) (03-20-2023)

excellent

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: AC16

GTPBP9 Antibody Western Blot validation (1000 dilution) in AC16 (Cat no:16371-1-AP)

FH

p (Verified Customer) (05-27-2021)

Good

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: H9C2

GTPBP9 Antibody Western Blot validation (1:3000 dilution) in H9C2 (Cat no:16371-1-AP)