Anticorps Polyclonal de lapin anti-OLFM3

OLFM3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12596-1-PBS

Synonymes

Olfactomedin 3, NOE3, Noelin 3, NOELIN3, Noelin-3

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Galerie de données de validation

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  WB analysis of PC-3 using 12596-1-AP (same clone as 12596-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12596-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 12596-1-AP (same clone as 12596-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12596-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 12596-1-AP (same clone as 12596-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12596-1-PBS in a different storage buffer formulation.

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  WB analysis of A2780 using 12596-1-AP (same clone as 12596-1-PBS)

  A2780 cells were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12596-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 12596-1-AP (same clone as 12596-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12596-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 12596-1-AP (same clone as 12596-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12596-1-AP (OLFM3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12596-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 12596-1-AP (same clone as 12596-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12596-1-AP (OLFM3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12596-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12596-1-AP (same clone as 12596-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using OLFM3 antibody (12596-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12596-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12596-1-PBS cible OLFM3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OLFM3 Protéine recombinante Ag3207
• Nom complet: olfactomedin 3
• Masse moléculaire calculée: 458 aa, 53 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC022531
• Symbole du gène: OLFM3
• Identification du gène (NCBI): 118427
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

OLFM3, also known as Noelin-3, belongs to a larger family of proteins containing the olfactomedin domain. OLFM3, which is most closely related to OLFM2, is expressed in the retinal ganglion and inner nuclear layers of the adult rat retina (PMID: 12019210).

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