Anticorps Polyclonal de lapin anti-OLIG1

OLIG1 Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 26763-1-PBS

Synonymes

Oligo1, Class E basic helix-loop-helix protein 21, Class B basic helix-loop-helix protein 6, bHLHe21, bHLHb6

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Galerie de données de validation

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  WB analysis using 26763-1-AP (same clone as 26763-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26763-1-AP (OLIG1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26763-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 26763-1-AP (same clone as 26763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26763-1-AP (OLIG1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26763-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 26763-1-AP (same clone as 26763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26763-1-AP (OLIG1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26763-1-PBS in a different storage buffer formulation.

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Informations sur le produit

26763-1-PBS cible OLIG1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OLIG1 Protéine recombinante Ag25128
• Nom complet: oligodendrocyte transcription factor 1
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC026989
• Symbole du gène: OLIG1
• Identification du gène (NCBI): 116448
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The oligodendrocyte lineage-specific basic helix-loop-helix (OLIG) family of transcription factors includes OLIG1-OLIG3, which differ in tissue expression. OLIG1 and OLIG2 are specifically expressed in nervous tissue as gene regulators of oligodendrogenesis. OLIG1 and OLIG2 interact with the Nkx-2.2 homeodomain protein, which is responsible for directing ventral neuronal patterning in response to graded Sonic hedgehog signaling in the embryonic neural tube. These interactions between OLIG proteins and Nkx-2.2 appear to promote the formation of alternate cell types by inhibiting V3 interneuron development. OLIG1 and OLIG2 are abundantly expressed in oligodendroglioma and nearly absent in astrocytomas. Therefore, OLIG proteins are candidates for molecular markers of human glial brain tumors, which are the most common primary malignancies of the human brain.

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