Anticorps Polyclonal de lapin anti-OLIG2

OLIG2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

60

Avis (4)

N° de cat : 13999-1-AP

Synonymes

BHLHB1, bHLHe19, OLIG2, OLIGO2, PRKCBP2, RACK17

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Galerie de données de validation

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  WB analysis using 13999-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13999-1-AP (OLIG2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13999-1-AP

  IP result of anti-OLIG2(IP:13999-1-AP, 4ug; Detection:13999-1-AP 1:1000) with mouse brain tissue lysate 1600 ug.

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  IHC staining of mouse brain using 13999-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13999-1-AP (OLIG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13999-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13999-1-AP (OLIG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13999-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13999-1-AP (OLIG2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13999-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13999-1-AP (OLIG2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13999-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13999-1-AP (OLIG2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 13999-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 13999-1-AP (OLIG2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cerveau de rat
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral de souris, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de rat,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below
• IHC: See 12 publications below
• IF: See 41 publications below

Informations sur le produit

13999-1-AP cible OLIG2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OLIG2 Protéine recombinante Ag5089
• Nom complet: oligodendrocyte lineage transcription factor 2
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32-36 kDa
• Numéro d’acquisition GenBank: BC047511
• Symbole du gène: OLIG2
• Identification du gène (NCBI): 10215
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

What is the specificity of Olig2?

Oligodendrocyte transcription factor 2 (OLIG2) is expressed by cells found in the central nervous system (CNS) called oligodendrocyte precursor cells (OPCs), which form the myelin sheaths wrapping the axons of neurons in the brain and spinal cord. OPCs differentiate into oligodendrocytes that form the myelin, providing metabolic support and saltatory conduction. OLIG2 is expressed broadly throughout their development to OPCs. This protein can be used to identify many cells of the oligodendrocyte lineage. It is found mostly in the nucleoplasm but also in the cytoplasm.

What is the function of OLIG2?

The basic helix-loop-helix structure of OLIG2 allows it to function as a transcription factor, determining cell fate in the development of neural tissue, where it is located at the pMN domain in the embryonic spinal cord. Expression of OLIG2 causes neural precursors to develop into oligodendrocytes or into motor neurons and expression is then maintained postnatally. OLIG2 co-operates with other factors to cause this differentiation from precursors, although overexpression alone can cause differentiation to the oligodendrocyte lineage.¹ The continued expression of OLIG2 in OPCs indicates an ongoing role in the maintenance of their stemness.

What is the involvement of OLIG2 in disease?

The expression of OLIG2 in glioblastoma, the most common type of malignant brain tumor in adults, is well characterized, where the pathological function is an extension of the normal function. Stem-like cells that propagate the tumor growth have been shown to be OLIG2-expressing, and are one of the key transcription factors involved in the re-programming of differentiated cells of the tumor to stem-like cells.² OLIG2 has also been associated with demyelinating diseases like multiple sclerosis (MS), as OPCs have been shown to be involved in the process of remyelination.³

1.     Liu, Z. et al. Induction of oligodendrocyte differentiation by Olig2 and Sox10: Evidence for reciprocal interactions and dosage-dependent mechanisms. Dev. Biol. 302, 683-693 (2007).

2.     Wegener, A. et al. Gain of Olig2 function in oligodendrocyte progenitors promotes remyelination. Brain 138, 120-35 (2015).

3.     Ettle, B., Schlachetzki, J. C. M. & Winkler, J. Oligodendroglia and Myelin in Neurodegenerative Diseases: More Than Just Bystanders? Mol. Neurobiol. 53, 3046-3062 (2016). 

Publications

Species	Application	Title
mouse	IF	Nature; Suppression of proteolipid protein rescues Pelizaeus-Merzbacher disease.; Authors - Matthew S Elitt
mouse	IF	Nat Biomed Eng; Variants of the adeno-associated virus serotype 9 with enhanced penetration of the blood-brain barrier in rodents and primates; Authors - Yizheng Yao
mouse	IHC	Cell Stem Cell; Non-canonical Targets of HIF1a Impair Oligodendrocyte Progenitor Cell Function.; Authors - Kevin C Allan
mouse	WB	J Pineal Res; Melatonin pretreatment alleviates the long-term synaptic toxicity and dysmyelination induced by neonatal Sevoflurane exposure via MT1 receptor-mediated Wnt signaling modulation.; Authors - Lirong Liang
human		Neuron; Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration.; Authors - Geneva R LaForce
mouse	IF	Neuron; Macrophages Expressing GALC Improve Peripheral Krabbe Disease by a Mechanism Independent of Cross-Correction.; Authors - Nadav I Weinstock

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Reyes (Verified Customer) (03-01-2024)

Olig2 (in red) marked the nuclei of tiny cells (oligodendrocytes) around the neurons (in green)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

OLIG2 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:13999-1-AP)

FH

Clarisse (Verified Customer) (08-29-2022)

This antibody works very well (clear nuclear signal).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human cerebral organoids

OLIG2 Antibody Immunofluorescence validation (1:500 dilution) in Human cerebral organoids (Cat no:13999-1-AP)

FH

An (Verified Customer) (09-17-2020)

Tried a free aliquot of this antibody to perform IHC on retinal cryosections of zebrafish. Did not work, although it was to be expected as the sequence homology was not great.

• Applications: Immunohistochemistry

FH

Alexander (Verified Customer) (08-05-2019)

This is a great product that has a clear nuclear stain at a 1:200 dilution and 1 hour primary incubation at room temp and 1 hour secondary incubation at room temp.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: brain organoid/neural progenitor cells