Anticorps Polyclonal de lapin anti-OLIG2

OLIG2 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 25754-1-AP

Synonymes

BHLHB1, bHLHe19, OLIG2, OLIGO2, PRKCBP2, RACK17

---

Galerie de données de validation

• 

  WB analysis of rat brain using 25754-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25754-1-AP (OLIG2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of C6 using 25754-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 25754-1-AP (OLIG2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IHC staining of human gliomas using 25754-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25754-1-AP (OLIG2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 25754-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25754-1-AP (OLIG2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 25754-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25754-1-AP (OLIG2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 25754-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25754-1-AP (OLIG2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25754-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25754-1-AP (OLIG2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25754-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25754-1-AP (OLIG2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu cérébral de rat, cellules C6
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral de souris, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

25754-1-AP cible OLIG2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OLIG2 Protéine recombinante Ag22642
• Nom complet: oligodendrocyte lineage transcription factor 2
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC047511
• Symbole du gène: OLIG2
• Identification du gène (NCBI): 10215
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The oligodendrocyte lineage-specific basic helix-loop-helix (OLIG) family of transcription factors include OLIG1-OLIG3, which differ in tissue expression. OLIG1 and OLIG2 are specifically expressed in nervous tissue as gene regulators of oligodendrogenesis. OLIG1 and OLIG2 interact with the Nkx-2.2 homeodomain protein, which is responsible for directing ventral neuronal patterning in response to graded Sonic hedgehog signaling in the embryonic neural tube. These interactions between OLIG proteins and Nkx-2.2 appear to promote the formation of alternate cell types by inhibiting V3 interneuron development. OLIG1 and OLIG2 are abundantly expressed in oligodendroglioma and nearly absent in astrocytomas. Therefore, OLIG proteins are candidates for molecular markers of human glial brain tumors, which are the most common primary malignancies of the human brain.