Anticorps Monoclonal anti-OLIG2 (for WB only)

OLIG2 (for WB only) Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

1E8C5

N° de cat : 66513-1-Ig

Synonymes

BHLHB1, bHLHe19, OLIG2, OLIGO2, PRKCBP2, RACK17

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Galerie de données de validation

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  WB analysis of human brain using 66513-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66513-1-Ig (OLIG2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human cerebellum using 66513-1-Ig

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66513-1-Ig (OLIG2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, tissu de cervelet humain

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

66513-1-Ig cible OLIG2 (for WB only) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: OLIG2 (for WB only) Protéine recombinante Ag18838
• Nom complet: oligodendrocyte lineage transcription factor 2
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 35-39 kDa
• Numéro d’acquisition GenBank: BC047511
• Symbole du gène: OLIG2
• Identification du gène (NCBI): 10215
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

OLIG2, also named as BHLHB1, BHLHE19, PRKCBP2 and RACK17, is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1, OLIG2 establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. OLIG2 is widely expressed in subsets of glia cells and progenitors, and it is strongly induced at different sites by both acute and chronic injury, albeit with different mechanisms. OLIG2 acts as a repressor of neurogenesis in cells reacting to brain injury. It may represent an effective approach towards evoking neuronal repair from parenchymal precursors.(PMID:19390819 )

Publications

Species	Application	Title
human	WB	Front Oncol; Prognostic Value and Biological Function of Galectins in Malignant Glioma.; Authors - Hongtao Zhu
human	WB	Nucleic Acids Res; A bio-orthogonal linear ubiquitin probe identifies STAT3 as a direct substrate of OTULIN in glioblastoma; Authors - Xianli Du