Anticorps Recombinant de lapin anti-OLIG2

OLIG2 Recombinant Antibody for WB, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

240839C1

N° de cat : 82806-7-RR

Synonymes

240839C1, 5O7, bHLHb1, bHLHe19, Class B basic helix-loop-helix protein 1

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Galerie de données de validation

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  WB analysis using 82806-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82806-7-RR (OLIG2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using 82806-7-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using OLIG2 antibody (82806-7-RR, Clone: 240839C1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 82806-7-RR

  Biolayer interferometry (BLl) kinetic assays of 82806-7-RR against Human OLIG2 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules THP-1, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82806-7-RR cible OLIG2 dans les applications de WB, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: OLIG2 Protéine recombinante Ag5089
• Nom complet: oligodendrocyte lineage transcription factor 2
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC047511
• Symbole du gène: OLIG2
• Identification du gène (NCBI): 10215
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

OLIG2, also named as BHLHB1, BHLHE19, PRKCBP2 and RACK17, is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1, OLIG2 establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. OLIG2 is widely expressed in subsets of glia cells and progenitors, and it is strongly induced at different sites by both acute and chronic injury, albeit with different mechanisms. OLIG2 acts as a repressor of neurogenesis in cells reacting to brain injury. It may represent an effective approach towards evoking neuronal repair from parenchymal precursors.(PMID:19390819 )

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