Anticorps Polyclonal de lapin anti-ONECUT2

• Phare
• Validé par KD/KO

ONECUT2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21916-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 21916-1-AP (same clone as 21916-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21916-1-PBS in a different storage buffer formulation.

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  WB analysis using 21916-1-AP (same clone as 21916-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21916-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skin using 21916-1-AP (same clone as 21916-1-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21916-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 21916-1-AP (same clone as 21916-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21916-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 21916-1-AP (same clone as 21916-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21916-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 21916-1-AP (same clone as 21916-1-PBS)

  IP result of anti-ONECUT2 (IP:21916-1-AP, 4ug; Detection:21916-1-AP 1:300) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 21916-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 21916-1-AP (same clone as 21916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21916-1-AP (ONECUT2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21916-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21916-1-PBS cible ONECUT2 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ONECUT2 Protéine recombinante Ag16162
• Nom complet: one cut homeobox 2
• Masse moléculaire calculée: 504 aa, 54 kDa
• Poids moléculaire observé: 54-60 kDa
• Numéro d’acquisition GenBank: BC108925
• Symbole du gène: ONECUT2
• Identification du gène (NCBI): 9480
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ONECUT2, also named as One cut domain family member 2 or Hepatocyte nuclear factor 6-beta, is a 504 amino acid protein, which contains one CUT DNA-binding domain and one homeobox DNA-binding domain. ONECUT2 belongs to the CUT homeobox family and activates the transcription of a number of liver genes such as HNF3B. ONECUT2 is required for liver differnetiation and metabolism. ONECUT2 and ONECUT3 play redundant roles with ONECUT1 in pancreas development.

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