Anticorps Monoclonal anti-OPA1

• Phare
• Validé par KD/KO

OPA1 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1B2D8

N° de cat : 66583-1-PBS

Synonymes

FLJ12460, KIAA0567, largeG, MGM1, NTG, OPA1, Optic atrophy protein 1

---

Galerie de données de validation

• 

  WB analysis using 66583-1-Ig (same clone as 66583-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66583-1-Ig (OPA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66583-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66583-1-Ig (same clone as 66583-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66583-1-Ig (OPA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66583-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66583-1-Ig (same clone as 66583-1-PBS)

  WB result of OPA1 antibody (66583-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-OPA1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66583-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66583-1-Ig (same clone as 66583-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66583-1-Ig (OPA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66583-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66583-1-Ig (same clone as 66583-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66583-1-Ig (OPA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66583-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66583-1-PBS cible OPA1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: OPA1 Protéine recombinante Ag26868
• Nom complet: optic atrophy 1 (autosomal dominant)
• Masse moléculaire calculée: 960 aa, 112 kDa
• Poids moléculaire observé: 100 kDa and 80-90 kDa
• Numéro d’acquisition GenBank: BC075805
• Symbole du gène: OPA1
• Identification du gène (NCBI): 4976
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

OPA1 is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. OPA1 localizes to the inner mitochondrial membrane and helps regulate mitochondrial stability and energy output. This protein also sequesters cytochrome c. OPA1 is associated with the inner membrane and protects cells from apoptosis by regulating inner membrane dynamics. Mutation of OPA1 causes the disease dominant optic atrophy, a degeneration of the retinal ganglion cells. OPA1 undergoes complex posttranscriptional regulation and posttranslational proteolysis. OPA1 is regulated by proteolytic cleavage, which degrades long OPA1 isoforms into short isoforms. The gene OPA1 can be cleaved into some chains with MW 100 kDa and 80-90 kDa.

{{ptg:RelatedPrimaryAntibodies}}