Anticorps Polyclonal de lapin anti-Oligophrenin 1

Oligophrenin 1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 11076-1-AP

Synonymes

MRX60, oligophrenin 1, OPHN1, OPN1

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Galerie de données de validation

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  WB analysis of HeLa using 11076-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11076-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11076-1-AP

  IP result of anti-Oligophrenin 1 (IP:11076-1-AP, 4ug; Detection:11076-1-AP 1:300) with mouse brain tissue lysate 4000 ug.

• 

  IHC staining of human cerebellum using 11076-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cerebellum using 11076-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human retinoblastoma using 11076-1-AP

  Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human retinoblastoma using 11076-1-AP

  Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human retinoblastoma using 11076-1-AP

  Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human retinoblastoma using 11076-1-AP

  Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11076-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de cervelet humain, tissu de rétinoblastome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below

Informations sur le produit

11076-1-AP cible Oligophrenin 1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Oligophrenin 1 Protéine recombinante Ag1536
• Nom complet: oligophrenin 1
• Masse moléculaire calculée: 802 aa, 92 kDa
• Poids moléculaire observé: 92 kDa
• Numéro d’acquisition GenBank: BC059393
• Symbole du gène: OPHN1
• Identification du gène (NCBI): 4983
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The OPHN1 gene encodes a Rho-GTPase activating protein (RhoGAP), and mutations in OPHN1 are responsible for non-specific X-linked mental retardation (NSMR). OPHN1 is highly expressed in the brain and present both pre- and postsynaptically in neurons.

Publications

Species	Application	Title
human	IHC	Ann Surg Oncol; Additive lymph node dissection may be necessary in minute submucosal cancer of the stomach after endoscopic resection.; Authors - Kim Jie-Hyun JH
human	WB	PLoS One; Oligophrenin-1 (OPHN1), a gene involved in X-linked intellectual disability, undergoes RNA editing and alternative splicing during human brain development.; Authors - Sabina Barresi
mouse	WB,IHC	Gene Expr Patterns; Oligophrenin-1 (Ophn1) is expressed in mouse retinal vessels.; Authors - Sel Saadettin S
mouse	WB	Neurosci Lett; Alpha lipoic acid treatment in late middle age improves cognitive function: Proteomic analysis of the protective mechanisms in the hippocampus; Authors - Jian Zhang