Anticorps Recombinant de lapin anti-Osteopontin

Osteopontin Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

6B11

N° de cat : 80912-4-RR

Synonymes

OPN, SPP1, SPP1, 6B11, BNSP, Bone sialoprotein 1

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Galerie de données de validation

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  WB analysis using 80912-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80912-4-RR (OPN, SPP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using 80912-4-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug OPN, SPP1 Recombinant antibody (80912-4-RR, Clone:6B11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 80912-4-RR

  Biolayer interferometry (BLl) kinetic assays of 80912-4-RR against Human OPN, SPP1 were performed. The affinity constant is 199 nM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules C2C12, cellules HepG2, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

80912-4-RR cible Osteopontin dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Osteopontin Protéine recombinante Ag19216
• Nom complet: secreted phosphoprotein 1
• Masse moléculaire calculée: 314 aa, 35 kDa
• Poids moléculaire observé: 70 kDa, 44-66 kDa
• Numéro d’acquisition GenBank: BC007016
• Symbole du gène: Osteopontin
• Identification du gène (NCBI): 6696
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidneys, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands corresponding to peptide fragments (PMID: 8195113; 17890765).

Publications

Species	Application	Title
human	WB	Cell Death Dis; LINC01133 promotes pancreatic ductal adenocarcinoma epithelial-mesenchymal transition mediated by SPP1 through binding to Arp3; Authors - Yefan Yang
human	WB	J Neurooncol; Exploring the prognostic value of BRMS1 + microglia based on single-cell anoikis regulator patterns in the immunologic microenvironment of GBM; Authors - Songyun Zhao
human	WB	IUBMB Life; Modulation of Osteogenic Differentiation by CYBB in Osteoporotic Models; Authors - Zhaodong Wang

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