Anticorps Polyclonal de lapin anti-ORAI1

• Phare
• Validé par KD/KO

ORAI1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(11)

Avis (1)

N° de cat : 28411-1-AP

Synonymes

ORAI1-L2, Orail-L2, Orail L2, ORAI1 L2, ORAI1 L1

---

Galerie de données de validation

• 

  WB analysis using 28411-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 28411-1-AP (ORAI1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28411-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 28411-1-AP (ORAI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 28411-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28411-1-AP (ORAI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 28411-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28411-1-AP (ORAI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules MDA-MB-453s, cellules MCF-7
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IF: See 4 publications below

Informations sur le produit

28411-1-AP cible ORAI1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ORAI1 Protéine recombinante Ag29234
• Nom complet: ORAI calcium release-activated calcium modulator 1
• Masse moléculaire calculée: 301 aa, 33 kDa
• Poids moléculaire observé: 45-55 kDa
• Numéro d’acquisition GenBank: BC015369
• Symbole du gène: ORAI1
• Identification du gène (NCBI): 84876
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. ORAI1 is a complex glycosylated protein, it exists in several molecular weight forms: a theoretical molecular weight of 32.7 kDa of the unmodified protein and two major glycosylated proteins of ~43 kDa and ~50 kDa (PMID: 22318387). Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.

Publications

Species	Application	Title
human	WB	Front Oncol; Calcium Channel Protein ORAI1 Mediates TGF-β Induced Epithelial-to-Mesenchymal Transition in Colorectal Cancer Cells.; Authors - Qingjie Kang ; KD Validated
human	WB	J Mol Med (Berl); Phosphate-induced ORAI1 expression and store-operated Ca2+ entry in aortic smooth muscle cells.; Authors - Ke Ma
mouse	WB,IF	Life Sci; The role of STIM1/ORAI1 channel in the analgesic effect of grain-sized moxibustion on inflammatory pain mice model.; Authors - Cheng-Shun Zhang
bovine	WB	J Dairy Sci; Role of ORAI calcium release-activated calcium modulator 1 (ORAI1) on neutrophil extracellular trap formation in dairy cows with subclinical hypocalcemia.; Authors - Bingbing Zhang ; KD Validated
bovine	IF	J Anim Sci; Intracellular Ca2+ signaling and ORAI calcium release-activated calcium modulator 1 (ORAI1) are associated with hepatic lipidosis in dairy cattle.; Authors - Ming Li
mouse	WB	Exp Lung Res; Store-operated Ca2+ entry contributes to the ASM phenotype transition in asthma; Authors - Hangqi Ni

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

R. (Verified Customer) (09-16-2024)

Results were spotty. Some non-specific binding occurred.

• Applications: Western Blot

{{ptg:RelatedPrimaryAntibodies}}