Anticorps Polyclonal de lapin anti-ORAI3

ORAI3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (1)

N° de cat : 25766-1-AP

Synonymes

Protein orai-3, TMEM142C, Transmembrane protein 142C

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Galerie de données de validation

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  WB analysis using 25766-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25766-1-AP (ORAI3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 25766-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25766-1-AP (ORAI3 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of human lung cancer using 25766-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25766-1-AP (ORAI3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules BxPC-3, cellules LNCaP, cellules PC-3
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

25766-1-AP cible ORAI3 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ORAI3 Protéine recombinante Ag22513
• Nom complet: ORAI calcium release-activated calcium modulator 3
• Masse moléculaire calculée: 295 aa, 32 kDa
• Poids moléculaire observé: ~30 kDa, 60 kDa
• Numéro d’acquisition GenBank: BC006126
• Symbole du gène: ORAI3
• Identification du gène (NCBI): 93129
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ORAI3, also named TMEM142C, is a key regulator or component of store-operated Ca2+ channels and transcription factor NFAT nuclear import. ORAI3 channels appeared to differ from Orai1 and -2 in being somewhat resistant to Ca2+ depotentiation. The molecular weight of monomers and dimers for Orai3 is about 30-35 kDa and 60-70 kDa (PMID: 20971921; 17293345; 22580508).

Publications

Species	Application	Title
human	WB	Sci Signal; ALOX5 drives the pyroptosis of CD4+ T cells and tissue inflammation in rheumatoid arthritis; Authors - Hao Cai
human	WB,IF,CoIP	BMJ Open Diabetes Res Care; Orai-IGFBP3 signaling complex regulates high-glucose exposure-induced increased proliferation, permeability, and migration of human coronary artery endothelial cells.; Authors - Suwen Bai
mouse	IP	BMJ Open Diabetes Res Care; Orai-vascular endothelial-cadherin signaling complex regulates high-glucose exposure-induced increased permeability of mouse aortic endothelial cells.; Authors - Yuan Wei
human	WB	Exp Ther Med; Resveratrol sensitizes A549 cells to irradiation damage via suppression of store-operated calcium entry with Orai1 and STIM1 downregulation.; Authors - Wu Lele
rat	WB	Allergy Asthma Immunol Res; Exercise Reduces Airway Smooth Muscle Contraction in Asthmatic Rats via Inhibition of IL-4 Secretion and Store-Operated Ca2+ Entry Pathway; Authors - Jun-Hao Huang
mouse	IF	J Orthop Translat; Tibial cortex transverse transport regulates Orai1/STIM1-mediated NO release and improve the migration and proliferation of vessels via increasing osteopontin expression; Authors - Lingchao Kong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

David (Verified Customer) (02-06-2023)

Works pretty well for our WB

• Applications: Western Blot

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