Anticorps Recombinant de lapin anti-ORAI3

ORAI3 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241568A2

N° de cat : 84340-2-PBS

Synonymes

Transmembrane protein 142C, TMEM142C, Protein orai-3, 241568A2

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Galerie de données de validation

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  WB analysis using 84340-2-RR (same clone as 84340-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84340-2-RR (ORAI3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84340-2-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 84340-2-RR (same clone as 84340-2-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 84340-2-RR (ORAI3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84340-2-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 84340-2-RR (same clone as 84340-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ORAI3 antibody (84340-2-RR, Clone: 241568A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84340-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01228-1

  Cytometric bead array standard curve of MP01228-1, ORAI3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84340-3-PBS. Detection antibody: 84340-2-PBS. Standard: Ag36135. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84340-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84340-2-RR against Human ORAI3 were performed. The affinity constant is 1.24 nM.

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Informations sur le produit

84340-2-PBS cible ORAI3 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ORAI3 Protéine recombinante Ag36135
• Nom complet: ORAI calcium release-activated calcium modulator 3
• Masse moléculaire calculée: 295 aa, 32 kDa
• Poids moléculaire observé: ~30 kDa，~60 kDa
• Numéro d’acquisition GenBank: BC006126
• Symbole du gène: ORAI3
• Identification du gène (NCBI): 93129
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ORAI3, also named TMEM142C, is a key regulator or component of store-operated Ca2+ channels and transcription factor NFAT nuclear import. ORAI3 channels appeared to differ from Orai1 and -2 in being somewhat resistant to Ca2+ depotentiation. The molecular weight of monomers and dimers for Orai3 is about 30-35 kDa and 60-70 kDa (PMID: 20971921; 17293345; 22580508).

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