Anticorps Polyclonal de lapin anti-ORM1

• Phare
• Validé par KD/KO

ORM1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16439-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 16439-1-AP (same clone as 16439-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16439-1-AP (ORM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse bladder using 16439-1-AP (same clone as 16439-1-PBS)

  mouse bladder tissue were subjected to SDS PAGE followed by western blot with 16439-1-AP (ORM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

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  IP experiment of human plasma using 16439-1-AP (same clone as 16439-1-PBS)

  IP result of anti-ORM1 (IP:16439-1-AP, 3ug; Detection:16439-1-AP 1:300) with human plasma lysate 100ug. This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 16439-1-AP (same clone as 16439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16439-1-AP (ORM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 16439-1-AP (same clone as 16439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16439-1-AP (ORM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 16439-1-AP (same clone as 16439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16439-1-AP (ORM1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 16439-1-AP (same clone as 16439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16439-1-AP (ORM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 16439-1-AP (same clone as 16439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16439-1-AP (ORM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 16439-1-AP (same clone as 16439-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ORM1 antibody (16439-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 16439-1-AP (same clone as 16439-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ORM1 Polyclonal antibody (16439-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16439-1-PBS cible ORM1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ORM1 Protéine recombinante Ag9758
• Nom complet: orosomucoid 1
• Masse moléculaire calculée: 201 aa, 24 kDa
• Poids moléculaire observé: 40-47 kDa
• Numéro d’acquisition GenBank: BC026238
• Symbole du gène: ORM1
• Identification du gène (NCBI): 5004
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Alpha-1-acid glycoprotein 1 (AGP1), also called orosomucoid-1 (ORM1), is a glycoprotein synthesized mostly by hepatocytes and present in human plasma. ORM1 is an acute-phase reactant protein controlled by glucocorticoids, interleukin-1 and interleukin-6, and increase up to 5-50 times upon infection and/or inflammation. Anti-apoptotic effect and role as immunomodulator of ORM have been reported. ORM is an important carrier for synthetic drugs and influences their distribution and availability in the body. This antibody recognizes a band about 44 kDa in human plasma which may be due to the glycosylation of ORM1 or the dimer formation of the protein.

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