Anticorps Polyclonal de lapin anti-ORP1L

ORP1L Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25268-1-PBS

Synonymes

ORP1, ORP 1, OSBPL1A, OSBPL1, OSBP8

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Galerie de données de validation

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  WB analysis using 25268-1-AP (same clone as 25268-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25268-1-AP (ORP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25268-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25268-1-PBS cible ORP1L dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: oxysterol binding protein-like 1A
• Masse moléculaire calculée: 108 kDa
• Poids moléculaire observé: 50 kDa, 105-110 kDa
• Numéro d’acquisition GenBank: NM_018030
• Symbole du gène: OSBPL1A
• Identification du gène (NCBI): 114876
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ORP1L (Oxysterol-binding protein-related protein 1L) is a lipid transfer protein that belongs to the family of OSBP-related proteins (ORPs). It is recruited to lysosomes via its ankyrin repeat domains that bind to Rab7(PMID: 34504082). ORP1L also possesses an FFAT motif, which allows it to interact with the ER resident VAP proteins to aid in establishing the ER-lysosomes contacts. ORP1L is expressed in various tissues, with particularly high expression in the brain, lungs, and macrophages. ORP1L plays a crucial role in cholesterol homeostasis. Disruption of ORP1L function can lead to a block in cholesterol transport to the ER, resulting in the accumulation of cholesterol-enriched LELs in the Golgi/perinuclear region. This can disrupt cellular cholesterol balance, potentially contributing to various metabolic diseases. This suggests that ORP1L is necessary for the normal functioning of cholesterol export from the endo-lysosomal system to the ER, and its dysfunction could be implicated in the progression of diseases related to cholesterol metabolism dysregulation(PMID: 28564600). The molecular weight of ORP1L is 50 kDa.

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