Anticorps Recombinant de lapin anti-OS9

• Phare
• Validé par KD/KO

OS9 Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230149B4

N° de cat : 82932-1-PBS

Synonymes

230149B4, Amplified in osteosarcoma 9, Protein OS-9

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Galerie de données de validation

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  WB analysis using 82932-1-RR (same clone as 82932-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82932-1-RR (OS9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82932-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 82932-1-RR (same clone as 82932-1-PBS)

  WB result of OS9 antibody (82932-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OS9 transfected MCF-7 cells. This data was developed using the same antibody clone with 82932-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 82932-1-RR (same clone as 82932-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82932-1-RR (OS9 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82932-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 82932-1-RR (same clone as 82932-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82932-1-RR (OS9 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82932-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82932-1-RR (same clone as 82932-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug OS9 Recombinant antibody (82932-1-RR, Clone:230149B4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82932-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82932-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82932-1-RR against Human OS9 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

82932-1-PBS cible OS9 dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: OS9 Protéine recombinante Ag0106
• Nom complet: osteosarcoma amplified 9, endoplasmic reticulum associated protein
• Masse moléculaire calculée: 76 kDa
• Poids moléculaire observé: 83-97 kDa
• Numéro d’acquisition GenBank: BC000532
• Symbole du gène: OS9
• Identification du gène (NCBI): 10956
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

OS-9 is a ubiquitously expressed ensoplasmic reticulum (ER)-associated protein originally identified as being amplified in certain osteosarcomas. It functions in ER quality control and ER-associated degradation (ERAD). There are three isoforms of this protein: OS-9.1, OS-9.2 and OS-9.3. The longest isoform, OS-9.1, contains 667 aa, OS-9.2 lacks aa 535-589, whereas OS-9.3 lacks aa 456-470 and 535-589. OS-9.1 and OS-9.2 are N-glycosylated, ubiquitously expressed in human tissues, and amplified in tumors.

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