Anticorps Polyclonal de lapin anti-Oncostatin M

Oncostatin M Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 27792-1-AP

Synonymes

OSM, Oncostatin-M

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Galerie de données de validation

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  WB analysis of MCF-7 using 27792-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27792-1-AP (Oncostatin M antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 27792-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27792-1-AP (Oncostatin M antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 27792-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 27792-1-AP (Oncostatin M antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 27792-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27792-1-AP (Oncostatin M antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 27792-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27792-1-AP (Oncostatin M antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 27792-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27792-1-AP (Oncostatin M antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 27792-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27792-1-AP (Oncostatin M antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules Jurkat, tissu testiculaire de rat
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

27792-1-AP cible Oncostatin M dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Oncostatin M Protéine recombinante Ag27093
• Nom complet: oncostatin M
• Masse moléculaire calculée: 252 aa, 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC011589
• Symbole du gène: OSM
• Identification du gène (NCBI): 5008
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Oncostatin M (OSM) is a multifunctional cytokine that belongs to the interleukin-6 (IL-6) family. It is primarily secreted by activated T lymphocytes and macrophages as a 28 kDa glycoprotein. OSM shares properties with all members of this cytokine family, but is most closely related in structure and function to leukemia inhibitory factor (LIF). OSM acts on a variety of cells and is involved in regulating gene activation, cell growth, and inflammatory processes. It is a potent inducer of anti-proteases, has anti-inflammatory properties, acts as an immune modifier, and promotes the deposition of extracellular matrix proteins . In the context of respiratory diseases, OSM may play a significant role in normal wound healing, as well as in remodeling and pathological fibrosis.

Publications

Species	Application	Title
human	IF	Jpn J Ophthalmol; Role of oncostatin M in the pathogenesis of vernal keratoconjunctivitis: focus on tissue remodeling.; Authors - Keitaro Mashimo
mouse	IHC	Int J Radiat Oncol Biol Phys; Oncostatin M/Oncostatin M Receptor Signal Induces Radiation-Induced Heart Fibrosis by Regulating SMAD4 in Fibroblast; Authors - Peng Xu
human	WB,IHC	Neoplasia; β-Catenin mediated TAM phenotype promotes pancreatic cancer metastasis via the OSM/STAT3/LOXL2 axis; Authors - Yijia Zhang

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