Anticorps Polyclonal de lapin anti-OSMR

• Phare
• Validé par KD/KO

OSMR Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, singe et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 10982-1-AP

Synonymes

IL 31 receptor subunit beta, IL 31R beta, IL 31R subunit beta, IL 31RB, oncostatin M receptor, OSMR, OSMR β, OSMRB, OSMRβ

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Galerie de données de validation

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  WB analysis using 10982-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10982-1-AP (OSMR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10982-1-AP

  WB result of OSMR antibody (10982-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-OSMR transfected HeLa cells.

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  WB analysis of A375 using 10982-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10982-1-AP (OSMR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 10982-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 10982-1-AP (OSMR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A375, cellules HEK-293T, cellules HepG2, cellules Raji

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

10982-1-AP cible OSMR dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, singe

• Réactivité: Humain, singe
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OSMR Protéine recombinante Ag1426
• Nom complet: oncostatin M receptor
• Masse moléculaire calculée: 110 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC010943
• Symbole du gène: OSMR
• Identification du gène (NCBI): 9180
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Oncostatin M (OSM) is a member of interleukin-6 family cytokines that modulates proliferation and differentiation in numerous cells (PMID: 22982441). OSM signals via a receptor complex comprising gp130 and the OSMR (also refered to as OSMRβ) (PMID: 8999038). OSMR is a single-pass membrane protein that belongs to the type I cytokine receptor family. OSMR mRNA is expressed at relatively high levels in all neural cells and fibroblast, epithelial, and various tumor cell lines (PMID: 8999038). It has also been shown that OSMR associates with IL31RA to form the heterodimeric IL31 receptor (PMID: 15184896). A 70 kDa soluble form can be detected by western blot (PMID: 17028186).

Publications

Species	Application	Title
human,mouse	WB	Cell Stem Cell; Oncostatin M is a major mediator of cardiomyocyte dedifferentiation and remodeling.; Authors - Kubin Thomas T
mouse,human	IF	Sci Adv; Repression of Osmr and Fgfr1 by miR-1/133a prevents cardiomyocyte dedifferentiation and cell cycle entry in the adult heart.; Authors - Melissa Valussi ; KO Validated
	IF	Br J Dermatol; CD11b+ cells markedly express the itch cytokine interleukin-31 in polymorphic light eruption.; Authors - V Patra
human	WB	Aging (Albany NY); Elevated lymphocyte specific protein 1 expression is involved in the regulation of leukocyte migration and immunosuppressive microenvironment in glioblastoma.; Authors - Jing-Yuan Cao
mouse	WB	Stem Cell Res Ther; Oncostatin M receptor regulates osteoblast differentiation via extracellular signal-regulated kinase/autophagy signaling.; Authors - Jie Zhou ; KD Validated
human	IHC	Acta Derm Venereol; IL-31 expression by inflammatory cells is preferentially elevated in atopic dermatitis.; Authors - Nobbe Stephan S

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