Anticorps Polyclonal de lapin anti-OTUB1

• Phare
• Validé par KD/KO

OTUB1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10573-1-PBS

Synonymes

EC:3.4.19.12, hOTU1, HSPC263, OTB1, OTU domain-containing ubiquitin aldehyde-binding protein 1

---

Galerie de données de validation

• 

  WB analysis using 10573-1-AP (same clone as 10573-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10573-1-AP (OTUB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10573-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 10573-1-AP (same clone as 10573-1-PBS)

  WB result of OTUB1 antibody (10573-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OTUB1 transfected HepG2 cells. This data was developed using the same antibody clone with 10573-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 10573-1-AP (same clone as 10573-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10573-1-AP (OTUB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10573-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 10573-1-AP (same clone as 10573-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using OTUB1 antibody (10573-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10573-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

10573-1-PBS cible OTUB1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OTUB1 Protéine recombinante Ag0845
• Nom complet: OTU domain, ubiquitin aldehyde binding 1
• Masse moléculaire calculée: 31 kDa and 35 kDa
• Poids moléculaire observé: 31-35 kDa
• Numéro d’acquisition GenBank: BC007519
• Symbole du gène: OTUB1
• Identification du gène (NCBI): 55611
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

OTUB1 belongs to the ovarian tumor domain protease (OTU) subfamily of deubiquitinases, which could block ubiquitination leading to protein stability. OTUB1 mainly prefers k-48 and k-63 ubiquitin chains type, especially in k-48 ubiquitin chain. OTUB1 performs its deubiquitinating function via two distinct manners: the canonical and non-canonical manner. OTUB1 is involved in many critical immune response processes, containing CD8 T cell activation, PD-L1 related immune evasion, and several infectious diseases. OTUB1 plays an important role in the occurrence, proliferation, invasion, and progression of various cancers, such as bladder cancer, breast cancer, prostate cancer, and colorectal cancer (PMID: 34875341, 35296795). OTUB1 has 2 isoforms with molecular weights of 31 and 35 kDa, respectively. With phospho or ubiquitin modification or reaction, the MW of OTUB1 will be increased.

{{ptg:RelatedPrimaryAntibodies}}