Anticorps Monoclonal anti-OTUB1
OTUB1 Monoclonal Antibody for IF/ICC
Hôte / Isotype
Mouse / IgG2a
Réactivité testée
Humain, rat, souris
Applications
IF/ICC
Conjugaison
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2F7F8
N° de cat : CL488-68489
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en IF/ICC | cellules U2OS, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Informations sur le produit
CL488-68489 cible OTUB1 dans les applications de IF/ICC et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris |
| Hôte / Isotype | Mouse / IgG2a |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | OTUB1 Protéine recombinante Ag33132 |
| Nom complet | OTU domain, ubiquitin aldehyde binding 1 |
| Masse moléculaire calculée | 31 kDa and 35 kDa |
| Poids moléculaire observé | 31-35 kDa |
| Numéro d’acquisition GenBank | BC007519 |
| Symbole du gène | OTUB1 |
| Identification du gène (NCBI) | 55611 |
| Conjugaison | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Forme | Liquide |
| Méthode de purification | Purification par protéine A |
| Tampon de stockage | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
| Conditions de stockage | Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
OTUB1 belongs to the ovarian tumor domain protease (OTU) subfamily of deubiquitinases, which could block ubiquitination leading to protein stability. OTUB1 mainly prefers k-48 and k-63 ubiquitin chains type, especially in k-48 ubiquitin chain. OTUB1 performs its deubiquitinating function via two distinct manners: the canonical and non-canonical manner. OTUB1 is involved in many critical immune response processes, containing CD8 T cell activation, PD-L1 related immune evasion, and several infectious diseases. OTUB1 plays an important role in the occurrence, proliferation, invasion, and progression of various cancers, such as bladder cancer, breast cancer, prostate cancer, and colorectal cancer (PMID: 34875341, 35296795). OTUB1 has 2 isoforms with molecular weights of 31 and 35 kDa, respectively.
Protocole
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 OTUB1 antibody CL488-68489 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
