Anticorps Polyclonal de lapin anti-OTUB2

• Phare
• Validé par KD/KO

OTUB2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12066-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 12066-1-AP (same clone as 12066-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12066-1-AP (OTUB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12066-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 12066-1-AP (same clone as 12066-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using OTUB2 antibody (12066-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 12066-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12066-1-PBS cible OTUB2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OTUB2 Protéine recombinante Ag2703
• Nom complet: OTU domain, ubiquitin aldehyde binding 2
• Masse moléculaire calculée: 234 aa, 27 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC009615
• Symbole du gène: OTUB2
• Identification du gène (NCBI): 78990
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

OTUB2, also known as C14orf137, belongs to the OTU family. OTUB2 is a functional cysteine protease with deubiquitinase activity. OTUB2 mediates the deubiquitination of substrate proteins, and the targets of substrate proteins can be diversified. Therefore, OTUB2 has a wide range of biological roles, including protecting against virus-induced activation of interferon regulatory factor 3 (IRF3) and NF-κB, promoting beta cell survival in human pancreatic islets, and supporting the DNA repair pathway (PMID: 30662561, 35309903). OTUB2 is widely expressed, with high expression levels in the brain. OTUB2 has 2 isoforms with molecular weights of 9 kDa and 27 kDa.

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