Anticorps Polyclonal de lapin anti-OTUD1

OTUD1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29921-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 29921-1-AP (same clone as 29921-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 29921-1-AP (OTUD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse colon using 29921-1-AP (same clone as 29921-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 29921-1-AP (OTUD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

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  WB analysis using 29921-1-AP (same clone as 29921-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29921-1-AP (OTUD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 29921-1-AP (same clone as 29921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 29921-1-AP (same clone as 29921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 29921-1-AP (same clone as 29921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 29921-1-AP (same clone as 29921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 29921-1-AP (same clone as 29921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29921-1-PBS cible OTUD1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OTUD1 Protéine recombinante Ag31996
• Nom complet: OTU domain containing 1
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 50-55 kDa
• Symbole du gène: OTUD1
• Identification du gène (NCBI): 220213
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

OTUD1, OTU domain-containing protein 1, is a deubiquitinating enzyme that specifically hydrolyzes 'Lys-63'-linked polyubiquitin to monoubiquitin (PMID: 23827681). OTUD1-mediated deubiquitination prevents activation of the ribosome quality control and subsequent dissociation of ribosomes and stimulates formation of polysomes and translation (PMID: 36445135). OTUD1 is a colon-specific deubiquitinase that expressed in normal colonic epithelium goblet cells (PMID: 36910614).

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