Anticorps Polyclonal de lapin anti-OTUD3

• Phare
• Validé par KD/KO

OTUD3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29622-1-PBS

Synonymes

DUBA4, KIAA0459, OTU domain containing 3, RP11 460G22.1

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Galerie de données de validation

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  WB analysis of HeLa using 29622-1-AP (same clone as 29622-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29622-1-AP (OTUD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29622-1-PBS in a different storage buffer formulation.

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  WB analysis of HCT 116 using 29622-1-AP (same clone as 29622-1-PBS)

  WB result of OTUD3 antibody (29622-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OTUD3 transfected HCT 116 cells. This data was developed using the same antibody clone with 29622-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 29622-1-AP (same clone as 29622-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29622-1-AP (OTUD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29622-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 29622-1-AP (same clone as 29622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29622-1-AP (OTUD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29622-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 29622-1-AP (same clone as 29622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29622-1-AP (OTUD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29622-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29622-1-PBS cible OTUD3 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OTUD3 Protéine recombinante Ag30425
• Nom complet: OTU domain containing 3
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: NM_015207
• Symbole du gène: OTUD3
• Identification du gène (NCBI): 23252
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

OTUD3 is a kind of deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. It's an important regulator of energy metabolism. Glucose and fatty acids trigger its nuclear translocation by CBP-dependent acetylation. In the nucleus, deubiquitinates and stabilizes the nuclear receptor PPARD regulating the expression of various genes involved in glucose and lipid metabolism and oxidative phosphorylation. The calculated MW of OTUD3 is 45 kDa, 29622-1-AP can detect the band around 43 kDa. (PubMed:23827681, 35675826).

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