Anticorps Recombinant de lapin anti-OTUD3

• Phare
• Validé par KD/KO

OTUD3 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242202B11

N° de cat : 84717-1-PBS

Synonymes

242202B11, DUBA4, KIAA0459, OTU domain containing 3, RP11 460G22.1

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Galerie de données de validation

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  WB analysis of HeLa using 84717-1-RR (same clone as 84717-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 84717-1-RR (OTUD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84717-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Neuro-2a using 84717-1-RR (same clone as 84717-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 84717-1-RR (OTUD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84717-1-PBS in a different storage buffer formulation.

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  WB analysis of HCT 116 using 84717-1-RR (same clone as 84717-1-PBS)

  WB result of OTUD3 antibody (84717-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OTUD3 transfected HCT 116 cells. This data was developed using the same antibody clone with 84717-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 84717-1-RR (same clone as 84717-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84717-1-RR (OTUD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84717-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01521-2

  Cytometric bead array standard curve of MP01521-2, OTUD3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84717-1-PBS. Detection antibody: 84717-2-PBS. Standard: Ag30425. Range: 0.781-50 ng/mL.

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  Affinity and Kinetic Characterization of 84717-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84717-1-RR against Human OTUD3 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84717-1-PBS cible OTUD3 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: OTUD3 Protéine recombinante Ag30425
• Nom complet: OTU domain containing 3
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: NM_015207
• Symbole du gène: OTUD3
• Identification du gène (NCBI): 23252
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

OTUD3 is a kind of deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. It's an important regulator of energy metabolism. Glucose and fatty acids trigger its nuclear translocation by CBP-dependent acetylation. In the nucleus, deubiquitinates and stabilizes the nuclear receptor PPARD regulating the expression of various genes involved in glucose and lipid metabolism and oxidative phosphorylation.

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