Anticorps Polyclonal de lapin anti-OTUD4

• Phare
• Validé par KD/KO

OTUD4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(16)

N° de cat : 25070-1-AP

Synonymes

DUBA6, HIN1, HIV 1 induced protein HIN 1, HSHIN1, KIAA1046, OTU domain containing 4, OTUD4

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Galerie de données de validation

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  WB analysis using 25070-1-AP

  Various lysate were subjected to SDS PAGE followed by western blot with 25070-1-AP (OTUD4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 25070-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25070-1-AP (OTUD4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 25070-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25070-1-AP (OTUD4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules Raji
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 15 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Informations sur le produit

25070-1-AP cible OTUD4 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OTUD4 Protéine recombinante Ag18401
• Nom complet: OTU domain containing 4
• Masse moléculaire calculée: 1113 aa, 124 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: BC118653
• Symbole du gène: OTUD4
• Identification du gène (NCBI): 54726
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

OTUD4, a member of the OTU family, encodes for a protein of 495 amino acids with an OTU domain and functions via their enzymatic deubiquitinating activities. OTUD4 nominally encodes a K48-specific deubiquitinase previously shown to counteract proteasomal degradation of DNA repair proteins. OTUD4 has been identified to be down-regulated in multiple human tumor types, and lower OTUD4 expression indicates a poor prognosis.(PMID: 36411454,PMID: 32184655,PMID: 29395066)

Publications

Species	Application	Title
human	WB	Nat Commun; Combinatorial ubiquitin code degrades deubiquitylation-protected substrates; Authors - Mai Morita
mouse	WB	Nat Commun; Osteocyte-derived extracellular vesicles mediate the bone-to-cartilage crosstalk and promote osteoarthritis progression; Authors - Na Liu
human	WB	Adv Sci (Weinh); Elevated SPARC Disrupts the Intestinal Barrier Integrity in Crohn's Disease by Interacting with OTUD4 and Activating the MYD88/NF-κB Pathway; Authors - Jiayu Wang
human	WB	Cell Death Differ; N6-methyladenosine-modified USP13 induces pro-survival autophagy and imatinib resistance via regulating the stabilization of autophagy-related protein 5 in gastrointestinal stromal tumors; Authors - Zhishuang Gao
human	WB	Front Microbiol; MiR-103a-3p Promotes Zika Virus Replication by Targeting OTU Deubiquitinase 4 to Activate p38 Mitogen-Activated Protein Kinase Signaling Pathway.; Authors - Haiyan Ye ; KD Validated
human	WB,IHC,IF,IP	Exp Cell Res; Deubiquitinating enzyme OTUD4 regulates metastasis in triple-negative breast cancer by stabilizing Snail1; Authors - Xiuqing Ma ; KD Validated

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