Anticorps Monoclonal anti-OTUD6B

OTUD6B Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D1G1

N° de cat : 68450-1-PBS

Synonymes

1D1G1, CGI 77, CGI-77, Deubiquitinase OTUD6B, DUBA 5

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Galerie de données de validation

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  WB analysis using 68450-1-Ig (same clone as 68450-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68450-1-Ig (OTUD6B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68450-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 68450-1-Ig (same clone as 68450-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using OTUD6B antibody (68450-1-Ig, Clone: 1D1G1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68450-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50962-1

  Cytometric bead array standard curve of MP50962-1, OTUD6B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68450-1-PBS. Detection antibody: 68450-2-PBS. Standard:Ag33607. Range: 0.098-50 ng/mL.

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  Cytometric bead array standard curve of MP50962-2

  Cytometric bead array standard curve of MP50962-2, OTUD6B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68450-1-PBS. Detection antibody: 68450-3-PBS. Standard:Ag33607. Range: 0.098-25 ng/mL.

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  Cytometric bead array standard curve of MP50962-3

  Cytometric bead array standard curve of MP50962-3, OTUD6B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68450-1-PBS. Detection antibody: 68450-4-PBS. Standard:Ag33607. Range: 0.098-12.5 ng/mL.

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Informations sur le produit

68450-1-PBS cible OTUD6B dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: OTUD6B Protéine recombinante Ag33607
• Nom complet: OTU domain containing 6B
• Masse moléculaire calculée: 293 aa, 34 kDa
• Poids moléculaire observé: 34-40 kDa
• Numéro d’acquisition GenBank: BC029760
• Symbole du gène: OTUD6B
• Identification du gène (NCBI): 51633
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

OTUD6B, also known as DUBA5, belongs to a DUB subfamily characterized by an ovarian tumor domain (OTU). It's a functional deubiquitinating enzyme, a class of protease that specifically cleaves ubiquitin linkages. OTUD6B function may be connected to growth and proliferation. It has been reported that OTUD6B could significantly suppress HCC cells migration and metastasis. The calculated molecular weight of OTUD6B is 34 kDa, 68450-1-Ig can detect the band around 34 kDa.(PMID:32323143)

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