Anticorps Polyclonal de lapin anti-OTUD7B

• Phare
• Validé par KD/KO

OTUD7B Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

17

Avis (1)

N° de cat : 16605-1-AP

Synonymes

CEZANNE, OTU domain containing 7B, OTU7B, OTUD7B, ZA20D1, Zinc finger protein Cezanne

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Galerie de données de validation

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  WB analysis of Jurkat using 16605-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16605-1-AP (OTUD7B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16605-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16605-1-AP (OTUD7B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 16605-1-AP

  IP Result of anti-OTUD7B (IP:16605-1-AP, 4ug; Detection:16605-1-AP 1:800) with Jurkat cells lysate 2500ug.

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  IHC staining of human kidney using 16605-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16605-1-AP (OTUD7B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 16605-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney using 16605-1-AP (OTUD7B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse kidney using 16605-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney using 16605-1-AP (OTUD7B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 16605-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16605-1-AP (OTUD7B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16605-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16605-1-AP (OTUD7B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 16605-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 16605-1-AP (OTUD7B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse heart using 16605-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 16605-1-AP (OTUD7B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16605-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16605-1-AP (OTUD7B antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque de souris, tissu cardiaque humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

16605-1-AP cible OTUD7B dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OTUD7B Protéine recombinante Ag9788
• Nom complet: OTU domain containing 7B
• Masse moléculaire calculée: 427aa,48 kDa; 843aa,93 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC020622
• Symbole du gène: OTUD7B
• Identification du gène (NCBI): 56957
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

OUTD7B is a member of the A20 family of the deubiquitinating cysteine proteases, which has deubiquitinating activity that is directed towards 'Lys-48' or 'Lys-63'-linked polyubiquitin chains. It plays a key role in the negative feedback regulation of NF-κB signaling in response to multiple stimuli and has been described as central gatekeeper in inflammation and immunity. The calculated molecular weight of OTUD7B is 93 kDa, but the modified OTUD7B is about 100 kDa.

Publications

Species	Application	Title
mouse	WB,IP	Nature; OTUD7B controls non-canonical NF-κB activation through deubiquitination of TRAF3.; Authors - Hu Hongbo H ; KO Validated
human	WB	Nat Commun; Dynamic ubiquitylation of Sox2 regulates proteostasis and governs neural progenitor cell differentiation.; Authors - Chun-Ping Cui ; KD Validated
mouse	WB	J Exp Med; Otud7b facilitates T cell activation and inflammatory responses by regulating Zap70 ubiquitination.; Authors - Hongbo Hu ; KO Validated
human	WB	Circ Res; Cezanne regulates inflammatory responses to hypoxia in endothelial cells by targeting TRAF6 for deubiquitination.; Authors - Luong Le A le A
mouse	IHC,WB,IF,CoIP	Theranostics; Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.; Authors - Gao-Shan Yang
mouse	WB,IF	Arterioscler Thromb Vasc Biol; Mechanical Activation of Hypoxia-Inducible Factor 1α Drives Endothelial Dysfunction at Atheroprone Sites.; Authors - Shuang Feng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Francesca (Verified Customer) (06-02-2019)

Good and specific antibody for Western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1: 2000 in 5% milk
• Cell Tissue Type: MeT-5A cells

OTUD7B Antibody Western Blot, validation (1: 2000 in 5% milk dilution) in MeT-5A cells (Cat no:16605-1-AP)