Anticorps Monoclonal anti-OTUD7B

• Phare
• Validé par KD/KO

OTUD7B Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

2C12D9

N° de cat : 66276-1-Ig

Synonymes

CEZANNE, OTU domain containing 7B, OTUD7B, ZA20D1, Zinc finger protein Cezanne

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Galerie de données de validation

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  WB analysis of Jurkat using 66276-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66276-1-Ig (OTUD7B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

66276-1-Ig cible OTUD7B dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: OTUD7B Protéine recombinante Ag10513
• Nom complet: OTU domain containing 7B
• Masse moléculaire calculée: 427aa,48 kDa; 843aa,93 kDa
• Poids moléculaire observé: 92 kDa
• Numéro d’acquisition GenBank: BC020622
• Symbole du gène: OTUD7B
• Identification du gène (NCBI): 56957
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

OUTD7B is a member of the A20 family of the deubiquitinating cysteine proteases, which has deubiquitinating activity that is directed towards 'Lys-48' or 'Lys-63'-linked polyubiquitin chains. It plays a key role in the negative feedback regulation of NF-κB signaling in response to multiple stimuli and has been described as central gatekeeper in inflammation and immunity.

Publications

Species	Application	Title
human	WB	Nature; TRAF2 and OTUD7B govern a ubiquitin-dependent switch that regulates mTORC2 signalling.; Authors - Bin Wang ; KD Validated
human	WB,IF	Autophagy; High-throughput screening of functional deubiquitinating enzymes in autophagy.; Authors - Shuo Tian ; KO Validated
human	WB,IF	Autophagy; OTUD7B deubiquitinates SQSTM1/p62 and promotes IRF3 degradation to regulate antiviral immunity.; Authors - Weihong Xie ; KD Validated
human	WB	Mol Biol Rep; DJ-1 activates the noncanonical NF-κB pathway via interaction with Cezanne to inhibit the apoptosis and promote the proliferation of Ishikawa cells; Authors - Qi‑Zhou Zhu ; KD Validated
human	IHC	J Exp Clin Cancer Res; OTUD7B suppresses Smac mimetic-induced lung cancer cell invasion and migration via deubiquitinating TRAF3.; Authors - Boxiang Zhang
human	WB, IHC	Cell Death Dis; OTUD7B stabilizes estrogen receptor α and promotes breast cancer cell proliferation.; Authors - Jianing Tang

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