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Anticorps Polyclonal de lapin anti-OTULIN

OTULIN Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27392-1-PBS

Synonymes

Galerie de données de validation

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  • Western Blot (WB) analysis of various lysates using OTULIN Polyclonal antibody (27392-1-AP)

    WB analysis using 27392-1-AP (same clone as 27392-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27392-1-AP (OTULIN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27392-1-PBS in a different storage buffer formulation.

Informations sur le produit

27392-1-PBS cible OTULIN dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène OTULIN Protéine recombinante Ag22302
Nom complet family with sequence similarity 105, member B
Masse moléculaire calculée 352 aa, 40 kDa
Poids moléculaire observé40-50 kDa
Numéro d’acquisition GenBankBC007706
Symbole du gène FAM105B
Identification du gène (NCBI) 90268
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Linear ubiquitination is negatively regulated by OTULIN (OTU deubiquitinase with linear linkage specificity, also known as Fam105b or Gumby), a deubiquitinase specifically catalysing the degradation of linear ubiquitin chains. OUb signalling is antagonised by deubiquitinases (DUBs), which cleave the polyUb signal from substrates to terminate signalling. OTU DUB with linear linkage specificity (OTULIN) and CYLD are the two main DUBs that regulate M1-polyUb signalling. OTULIN exclusively cleaves M1 linkages, whereas CYLD cleaves both M1 and K63 linkages. OTULIN binds directly to the LUBAC subunit HOIP and regulates LUBAC signalling, autoubiquitination, and stability.(PMID: 34625557, PMID: 32231246)

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