Anticorps Recombinant de lapin anti-OX40/TNFRSF4

OX40/TNFRSF4 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241398D11

N° de cat : 84247-2-PBS

Synonymes

TNFRSF4, ACT35, ACT35 antigen, CD134, OX40

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Galerie de données de validation

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  Sandwich ELISA sample test of MP01138-2

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean CD134/OX40 concentration was not determined in unstimulated PBMC supernatant, 232.1 pg/mL in PHA stimulated PBMC supernatant.

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  Sandwich ELISA sample test of MP01138-2

  The mean CD134/OX40 concentration was determined to be 4.0 ng/mL in Jurkat cell extract based on a 1.5 mg/mL extract load.

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  Sandwich ELISA sample test of MP01138-2

  Serum of six individual healthy human donors was measured. The CD134/OX40 concentration of detected samples was determined to be 780.8 pg/mL with a range of 152.2-1,428.1 pg/mL.

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  Sandwich ELISA standard curve of MP01138-2

  Sandwich ELISA standard curve of MP01138-2, Human CD134/OX40 Recombinant Matched Antibody Pair - PBS only. 84247-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1261. 84247-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Cytometric bead array standard curve of MP01138-2

  Cytometric bead array standard curve of MP01138-2, CD134/OX40 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84247-2-PBS. Detection antibody: 84247-1-PBS. Standard: Eg1261. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84247-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 84247-2-PBS against Human OX40/TNFRSF4 was performed. The affinity constant is 5.63 nM.

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Informations sur le produit

84247-2-PBS cible OX40/TNFRSF4 dans les applications de Cytometric bead array, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: OX40/TNFRSF4 Protéine recombinante Eg1261
• Nom complet: tumor necrosis factor receptor superfamily, member 4
• Masse moléculaire calculée: 29 kDa
• Numéro d’acquisition GenBank: NM_003327
• Symbole du gène: CD134
• Identification du gène (NCBI): 7293
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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