Anticorps Recombinant de lapin anti-OX40L/TNFSF4

OX40L/TNFSF4 Recombinant Antibody for WB, IHC, IF/ICC, FC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241813A6

N° de cat : 82794-7-PBS

Synonymes

OX40L, TNFSF4, TNFSF4/CD252, 241813A6, OX 40L

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Galerie de données de validation

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  WB analysis of HUVEC using 82794-7-RR (same clone as 82794-7-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 82794-7-RR (OX40L/TNFSF4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82794-7-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 82794-7-RR (same clone as 82794-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82794-7-RR (OX40L/TNFSF4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82794-7-PBS in a different storage buffer formulation.

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  IF Staining of MJ using 82794-7-RR (same clone as 82794-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MJ cells using OX40L/TNFSF4 antibody (82794-7-RR, Clone: 241813A6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82794-7-PBS in a different storage buffer formulation.

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  FC experiment of MJ using 82794-7-RR (same clone as 82794-7-PBS)

  1x10^6 MJ cells were surface stained with 0.25 ug OX40L/TNFSF4 Recombinant antibody (82794-7-RR, Clone:241813A6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were not fixed. This data was developed using the same antibody clone with 82794-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82794-7-RR

  Biolayer interferometry (BLl) kinetic assays of 82794-7-RR against Human OX40L/TNFSF4 were performed. The affinity constant is 13.8 pM.

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Informations sur le produit

82794-7-PBS cible OX40L/TNFSF4 dans les applications de WB, IHC, IF/ICC, FC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: OX40L/TNFSF4 Protéine recombinante Eg0582
• Nom complet: tumor necrosis factor (ligand) superfamily, member 4
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: NM_003326.5
• Symbole du gène: OX40L/TNFSF4
• Identification du gène (NCBI): 7292
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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