Anticorps Monoclonal anti-OXCT1

• Phare
• Validé par KD/KO

OXCT1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G1B9

N° de cat : 67836-1-PBS

Synonymes

1G1B9, 3 oxoacid CoA transferase 1, 3-oxoacid CoA-transferase 1, EC:2.8.3.5, OXCT

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Galerie de données de validation

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  WB analysis of rat heart using 67836-1-Ig (same clone as 67836-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

  WB result of OXCT1 antibody (67836-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXCT1 transfected HeLa cells. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit brain using 67836-1-Ig (same clone as 67836-1-PBS)

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67836-1-Ig (same clone as 67836-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 67836-1-Ig (same clone as 67836-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67836-1-Ig (same clone as 67836-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 67836-1-Ig (same clone as 67836-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 67836-1-Ig (same clone as 67836-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67836-1-Ig (OXCT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67836-1-Ig (same clone as 67836-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). CL594-Phalloidin (red). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human OXCT1 (67836-1-Ig, Clone:1G1B9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67836-1-PBS cible OXCT1 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: OXCT1 Protéine recombinante Ag24792
• Nom complet: 3-oxoacid CoA transferase 1
• Masse moléculaire calculée: 520 aa, 56 kDa
• Poids moléculaire observé: 52-56 kDa
• Numéro d’acquisition GenBank: BC009001
• Symbole du gène: OXCT1
• Identification du gène (NCBI): 5019
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

OXCT1 (also know as SCOT), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)

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