Anticorps Recombinant de lapin anti-OXCT1

• Phare
• Validé par KD/KO

OXCT1 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4K12

N° de cat : 81011-1-PBS

Synonymes

3 oxoacid CoA transferase 1, 3-oxoacid CoA-transferase 1, 4K12, EC:2.8.3.5, OXCT

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Galerie de données de validation

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  WB analysis of HeLa using 81011-1-RR (same clone as 81011-1-PBS)

  WB result of OXCT1 antibody (81011-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXCT1 transfected HeLa cells. This data was developed using the same antibody clone with 81011-1-PBS in a different storage buffer formulation.

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  WB analysis using 81011-1-RR (same clone as 81011-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81011-1-RR (OXCT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81011-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 81011-1-RR (same clone as 81011-1-PBS)

  WB result of OXCT1 antibody (81011-1-RR; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXCT1 transfected HeLa cells. This data was developed using the same antibody clone with 81011-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 81011-1-RR (same clone as 81011-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (81011-1-RR, Clone: 4K12 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81011-1-PBS in a different storage buffer formulation.

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  IF Staining of SKOV-3 using 81011-1-RR (same clone as 81011-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed SKOV-3 cells using OXCT1 antibody (81011-1-RR, Clone: 4K12 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81011-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 81011-1-RR (same clone as 81011-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (81011-1-RR, Clone: 4K12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81011-1-PBS in a different storage buffer formulation.

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Informations sur le produit

81011-1-PBS cible OXCT1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: OXCT1 Protéine recombinante Ag2818
• Nom complet: 3-oxoacid CoA transferase 1
• Masse moléculaire calculée: 520 aa, 56 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC009001
• Symbole du gène: OXCT1
• Identification du gène (NCBI): 5019
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)

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