Anticorps Polyclonal de lapin anti-OXER1

OXER1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55202-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of K-562 using 55202-1-AP (same clone as 55202-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 55202-1-AP (OXER1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55202-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 55202-1-AP (same clone as 55202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 55202-1-AP (OXER1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55202-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 55202-1-AP (same clone as 55202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 55202-1-AP (OXER1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 55202-1-AP (same clone as 55202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 55202-1-AP (OXER1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 55202-1-AP (same clone as 55202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 55202-1-AP (OXER1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55202-1-PBS in a different storage buffer formulation.

• 

  FC experiment of K-562 using 55202-1-AP (same clone as 55202-1-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.4 ug OXER1 Polyclonal antibody (55202-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 55202-1-PBS in a different storage buffer formulation.

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Informations sur le produit

55202-1-PBS cible OXER1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: oxoeicosanoid (OXE) receptor 1
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 43-46 kDa
• Numéro d’acquisition GenBank: NM_148962
• Symbole du gène: OXER1
• Identification du gène (NCBI): 165140
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

OXER1, also named as GPR170 and TG1019, is a receptor for eicosanoids and polyunsaturated fatty acids such as 5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid (5-OXO-ETE), 5(S)-hydroperoxy-6E,8Z,11Z,14Z-eicosatetraenoic acid (5(S)-HPETE) and arachidonic acid. It seems to be coupled to the G(i)/G(o), families of heteromeric G proteins. (PMID:12065583) This antibody is specific to OXER1.

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