Anticorps Recombinant de lapin anti-OXR1

OXR1 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

251027B10

N° de cat : 86359-3-RR

Synonymes

CHEGDD, Nbla00307, TLDC3

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Galerie de données de validation

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  WB analysis using 86359-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86359-3-RR (OXR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 86359-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86359-3-RR (OXR1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 86359-3-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 86359-3-RR (OXR1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 86359-3-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 86359-3-RR (OXR1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 86359-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86359-3-RR (OXR1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 86359-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86359-3-RR against Human OXR1 were performed. The affinity constant is 0.533 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules K-562, cellules Neuro-2a
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86359-3-RR cible OXR1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: OXR1 Protéine recombinante Ag4439
• Nom complet: oxidation resistance 1
• Masse moléculaire calculée: 758 aa, 85 kDa
• Poids moléculaire observé: 120-140 kDa
• Numéro d’acquisition GenBank: BC032710
• Symbole du gène: OXR1
• Identification du gène (NCBI): 55074
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Oxidation resistance protein 1 (OXR1) belongs to the OXR1 family. The major function of OXR1 is to control the expression of genes that alleviate oxidative stress by increasing cellular resistance to reactive oxygen species (ROS) and the stress these molecules cause the cell. OXR1's ability to reduce oxidative stress and neurodegeneration in multiple diseases strongly suggests that it can be an effective therapeutic target (PMID: 33384581). Biochemical experiments show that OXR1 inhibits V1-ATPase and causes disassembly of the holoenzyme, suggesting that OXR1 plays a direct role in V-ATPase regulation (PMID: 34918374). OXR1 has 8 isoforms with the molecular mass of 25, 28, 34, 56, 94, 95 and 98 kDa. Sometimes higher molecular weight around 120-140 kDa can also be observed, which may be a modified variant of OXR1.

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