Anticorps Polyclonal de lapin anti-OXTR

• Phare
• Validé par KD/KO

OXTR Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 23045-1-AP

Synonymes

OT R, OXTR, Oxytocin receptor

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Galerie de données de validation

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  WB analysis using 23045-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23045-1-AP (OXTR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 23045-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23045-1-AP (OXTR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 23045-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 23045-1-AP (Oxytocin Receptor antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23045-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23045-1-AP (Oxytocin Receptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 23045-1-AP

  IP Result of anti-OXTR (IP:23045-1-AP, 4ug; Detection:23045-1-AP 1:1000) with L02 cells lysate 1800ug.

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  IHC staining of human hysteromyoma using 23045-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 23045-1-AP (OXTR antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human hysteromyoma using 23045-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 23045-1-AP (OXTR antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 23045-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 23045-1-AP (Oxytocin Receptor antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 23045-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 23045-1-AP (Oxytocin Receptor antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of Jurkat using 23045-1-AP

  1X10^6 Jurkat cells were stained with 0.2ug OXTR antibody (23045-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules L02
• Résultats positifs en IP: cellules L02
• Résultats positifs en IHC: tissu d'hystéromyome humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 7 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Informations sur le produit

23045-1-AP cible OXTR dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OXTR Protéine recombinante Ag19074
• Nom complet: OXTR
• Masse moléculaire calculée: 389 aa, 43 kDa
• Poids moléculaire observé: 46 kDa, 67 kDa
• Numéro d’acquisition GenBank: BC137443
• Symbole du gène: OXTR
• Identification du gène (NCBI): 5021
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Oxytocin (OXT) is a neurohypophysial hormone that plays a role in lactation and parturition and in the central nervous system as a neurotransmitter involved in sex and maternal behavior (PMID: 1852313; 10811917). OXT acts through its receptor, OXTR, which belongs to the G protein-coupled 7-transmembrane receptor family. OXTR is expressed in endometrium, myometrium, mammary gland (PMID: 1313946). This antibody is raised against the C-terminal region of human OXTR. Two immunoreactive OXTR bands were found in the fat tissue, an unglycosylated 43 kDa form and a mature glycosylated 67 kDa form (PMID:32819381).

Publications

Species	Application	Title
mouse	WB,IF	Nat Commun; PMID: 24915299; Authors - Christian Elabd
mouse	IF	Acta Physiol (Oxf); Oxytocin modulates neural processing of mitral/tufted cells in the olfactory bulb.; Authors - Changcheng Sun
mouse	IF	Oxid Med Cell Longev; PMID: 32082478; Authors - Daniela C Wigger
mouse	IHC	Antioxidants (Basel); ΔMST and the Regulation of Cardiac CSE and OTR Expression in Trauma and Hemorrhage.; Authors - Britta Trautwein
human	WB	Biochem Pharmacol; Synergistic antitumor activity of 5-fluorouracil and atosiban against microsatellite stable colorectal cancer through restoring GATA3.; Authors - Mengmeng Wang ; KD Validated
human	WB	Front Pharmacol; The Role of Formyl Peptide Receptor 1 in Uterine Contraction During Parturition.; Authors - Chaolu Chen