Anticorps Polyclonal de lapin anti-Occludin

• Phare
• Validé par KD/KO

Occludin Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13409-1-PBS

Synonymes

OCLN

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Galerie de données de validation

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  WB analysis using 13409-1-AP (same clone as 13409-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 13409-1-AP (same clone as 13409-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 13409-1-AP (same clone as 13409-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 13409-1-AP (same clone as 13409-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human kidney using 13409-1-AP (same clone as 13409-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (OCLN antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 13409-1-AP (same clone as 13409-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (OCLN antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 13409-1-AP (same clone as 13409-1-PBS)

  IP result of anti-Occludin (IP:13409-1-AP, 3ug; Detection:13409-1-AP 1:500) with mouse liver tissue lysate 6400ug. This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13409-1-AP (same clone as 13409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13409-1-AP (same clone as 13409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 13409-1-AP (same clone as 13409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 13409-1-AP (same clone as 13409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 13409-1-AP (same clone as 13409-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Occludin antibody (13409-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 13409-1-AP (same clone as 13409-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Occludin antibody (13409-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13409-1-PBS cible Occludin dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Occludin Protéine recombinante Ag4057
• Nom complet: occludin
• Masse moléculaire calculée: 522 aa, 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC029886
• Symbole du gène: Occludin
• Identification du gène (NCBI): 4950
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

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