Anticorps Recombinant de lapin anti-Occludin

Occludin Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

8B2

N° de cat : 80545-1-PBS

Synonymes

OCLN, 8B2

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Galerie de données de validation

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  WB analysis using 80545-1-RR (same clone as 80545-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 80545-1-RR (Occludin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80545-1-RR (same clone as 80545-1-PBS)

  HaCaT cells were subjected to SDS PAGE followed by western blot with 80545-1-RR (Occludin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig colon using 80545-1-RR (same clone as 80545-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 80545-1-RR (Occludin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse colon using 80545-1-RR (same clone as 80545-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 80545-1-RR (Occludin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

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  IP experiment of HUVEC using 80545-1-RR (same clone as 80545-1-PBS)

  IP result of anti-Occludin (IP:80545-1-RR, 4ug; Detection:80545-1-RR 1:15000) with HUVEC cells lysate 1410 ug. This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 80545-1-RR (same clone as 80545-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Occludin antibody (80545-1-RR, Clone: 8B2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 80545-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 80545-1-RR against Human Occludin were performed. The affinity constant is 26.5 pM.

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Informations sur le produit

80545-1-PBS cible Occludin dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Occludin Protéine recombinante Ag26173
• Nom complet: occludin
• Masse moléculaire calculée: 522 aa, 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC029886
• Symbole du gène: Occludin
• Identification du gène (NCBI): 4950
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

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