Anticorps Polyclonal de lapin anti-Occludin

Occludin Polyclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications(3)

N° de cat : CL488-27260

Synonymes

OCLN

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Galerie de données de validation

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  IF Staining of MCF-7 using CL488-27260

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 Occludin antibody (CL488-27260) at dilution of 1:800.

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  IF Staining of Caco-2 using CL488-27260

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using CoraLite® Plus 488 Occludin antibody (CL488-27260) at dilution of 1:1000.

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  FC experiment of MCF-7 using CL488-27260

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Occludin (CL488-27260) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules MCF-7, cellules Caco-2
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 3 publications below

Informations sur le produit

CL488-27260 cible Occludin dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Occludin Protéine recombinante Ag26173
• Nom complet: occludin
• Masse moléculaire calculée: 522 aa, 59 kDa
• Numéro d’acquisition GenBank: BC029886
• Symbole du gène: Occludin
• Identification du gène (NCBI): 4950
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

Publications

Species	Application	Title
human	IF	J Neurodev Disord; Rescue of impaired blood-brain barrier in tuberous sclerosis complex patient derived neurovascular unit; Authors - Jacquelyn A Brown
mouse	IF	Cell; The choroid plexus synergizes with immune cells during neuroinflammation; Authors - Huixin Xu
	IF	Int J Pharm; A novel pH-responsive berberine-loaded attapulgite microsphere for IBD therapy via the regulation of gut immunity and flora; Authors - Shiwen Gong

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