Anticorps Polyclonal de lapin anti-Osteopontin

• Phare
• Validé par KD/KO

Osteopontin Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 30200-1-AP

Synonymes

OPN, 2AR, Bone sialoprotein 1, BSPI, Eta-1

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Galerie de données de validation

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  WB analysis using 30200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30200-1-AP (Osteopontin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 30200-1-AP

  WB result of Osteopontin antibody (30200-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Osteopontin transfected RAW 264.7 cells.

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  WB analysis using 30200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30200-1-AP (Osteopontin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30200-1-AP (Osteopontin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse stomach using 30200-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse stomach tissue using Osteopontin antibody (30200-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of RAW 264.7 using 30200-1-AP

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Osteopontin antibody (30200-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of RAW 264.7 using 30200-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using Osteopontin antibody (30200-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, tissu rénal de rat, tissu rénal humain
• Résultats positifs en IF-P: tissu d'estomac de souris,
• Résultats positifs en IF/ICC: cellules RAW 264.7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below

Informations sur le produit

30200-1-AP cible Osteopontin dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Osteopontin Protéine recombinante Eg0317
• Nom complet: secreted phosphoprotein 1
• Masse moléculaire calculée: 32kd
• Poids moléculaire observé: 32 kDa, 60-70 kDa
• Numéro d’acquisition GenBank: NM_001204233.1
• Symbole du gène: Osteopontin
• Identification du gène (NCBI): 20750
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Osteopontin (OPN) is also named as 2AR, minopontin, bone sialoprotein 1, Spp1, Eta-1 and Op, and belongs to the osteopontin family. Osteopontin is major non-collagenous bone protein that binds tightly to hydroxyapatite. It is a highly phosphorylated glycophosphoprotein having acidic characteristics and rich in aspartic acid (PMID: 30003880). And it acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (PMID:2351930, PMID:10657301). OPN, a multifunctional protein, has important functions on cardiovascular diseases, cancer, diabetes and kidney stone diseases and in the process of inflammation, biomineralization, cell viability and wound healing (PMID: 30003880). Osteopontin (OPN) is a pleiotropic glycoprotein expressed in various cell types in animals and in humans, including bone, immune, smooth muscle, epithelial and endothelial cells. Moreover, OPN is found in kidneys (in the thick ascending limbs of the loop of Henle and in distal nephrons) and urine (PMID:30456594). Osteopontin (OPN) is an acidic 60-70 kDa glycoprotein that is cleaved by proteases to yield 45-kDa and 24-kDa fragments (PMID: 10491621, PMID: 1995615)

Publications

Species	Application	Title
rat	WB	Int J Biol Macromol; Cranial repair-promoting effect of oxidised sodium alginate/amino gelatine injectable hydrogel loaded with deer antler blood peptides; Authors - Taojing Yu
human	WB	Biomedicines; Mechanistic Insights into Salvigenin for Glucocorticoid-Induced Femoral Head Osteonecrosis: A Network Pharmacology and Experimental Study; Authors - Zhengjie Zhu
rat,mouse	WB	J Transl Med; The surface protein Gbp of Fusobacterium nucleatum inhibits osteogenic differentiation by inactivating the Wnt/β-catenin pathway via binding to Annexin A2; Authors - Rui Dong
human	WB	J Gastrointest Oncol; SPP1 promotes tumor progression in esophageal carcinoma by activating focal adhesion pathway; Authors - Jianjun Sun
rat	WB	J Orthop Surg Res; Uniaxial static strain enhances osteogenic and angiogenic potential under hypoxic conditions in distraction osteogenesis; Authors - Lifang Zhang
mouse	IHC	Osteoarthritis Cartilage; M2 macrophages regulate nucleus pulposus cell extracellular matrix synthesis through the OPN-CD44 axis in intervertebral disc degeneration; Authors - Zhiwen Tao

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