Anticorps Recombinant de lapin anti-Osteopontin

Osteopontin Recombinant Antibody for IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

240250A1

N° de cat : 83413-6-RR

Synonymes

Spp1, 240250A1, Opn

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Galerie de données de validation

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  IF Staining of mouse stomach using 83413-6-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse stomach tissue using Osteopontin antibody (83413-6-RR, Clone: 240250A1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of RAW 264.7 using 83413-6-RR

  Immunofluorescent analysis of (4% PFA) fixed LPS and Brefeldin A treated RAW 264.7 cells using Osteopontin antibody (83413-6-RR, Clone: 240250A1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83413-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83413-6-RR against Mouse Osteopontin were performed. The affinity constant is 2.19 nM.

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Applications testées

• Résultats positifs en IF-P: tissu d'estomac de souris,
• Résultats positifs en IF/ICC: cellules RAW 264.7 traitées au LPS et à la bréfeldine A,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83413-6-RR cible Osteopontin dans les applications de IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Osteopontin Protéine recombinante Eg0317
• Nom complet: secreted phosphoprotein 1
• Masse moléculaire calculée: 32kd
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: NM_001204233.1
• Symbole du gène: Osteopontin
• Identification du gène (NCBI): 20750
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Osteopontin (OPN) is also named as 2AR, minopontin, bone sialoprotein 1, Spp1, Eta-1 and Op, and belongs to the osteopontin family. Osteopontin is major non-collagenous bone protein that binds tightly to hydroxyapatite. It is a highly phosphorylated glycophosphoprotein having acidic characteristics and rich in aspartic acid (PMID: 30003880). And it acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (PMID:2351930, PMID:10657301). OPN, a multifunctional protein, has important functions on cardiovascular diseases, cancer, diabetes and kidney stone diseases and in the process of inflammation, biomineralization, cell viability and wound healing (PMID: 30003880). Osteopontin (OPN) is a pleiotropic glycoprotein expressed in various cell types in animals and in humans, including bone, immune, smooth muscle, epithelial and endothelial cells. Moreover, OPN is found in kidneys (in the thick ascending limbs of the loop of Henle and in distal nephrons) and urine (PMID:30456594).

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