Anticorps Recombinant de lapin anti-Osteopontin

Osteopontin Recombinant Antibody for IF/ICC, IF-P

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

IF/ICC, IF-P

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240250A1

N° de cat : CL488-83413-6

Synonymes

Spp1, Opn, 240250A1

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Galerie de données de validation

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  IF Staining of mouse stomach using CL488-83413-6

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse stomach tissue using CoraLite® Plus 488 Osteopontin antibody (CL488-83413-6, Clone: 240250A1 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of RAW 264.7 using CL488-83413-6

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CoraLite® Plus 488 Osteopontin antibody (CL488-83413-6, Clone: 240250A1 ) at dilution of 1:200.

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Applications testées

• Résultats positifs en IF-P: tissu d'estomac de souris,
• Résultats positifs en IF/ICC: cellules RAW 264.7,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-83413-6 cible Osteopontin dans les applications de IF/ICC, IF-P et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: secreted phosphoprotein 1
• Masse moléculaire calculée: 32kd
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: NM_001204233.1
• Symbole du gène: Osteopontin
• Identification du gène (NCBI): 20750
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Osteopontin (OPN) is also named as 2AR, minopontin, bone sialoprotein 1, Spp1, Eta-1 and Op, and belongs to the osteopontin family. Osteopontin is major non-collagenous bone protein that binds tightly to hydroxyapatite. It is a highly phosphorylated glycophosphoprotein having acidic characteristics and rich in aspartic acid (PMID: 30003880). And it acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (PMID:2351930, PMID:10657301). OPN, a multifunctional protein, has important functions on cardiovascular diseases, cancer, diabetes and kidney stone diseases and in the process of inflammation, biomineralization, cell viability and wound healing (PMID: 30003880). Osteopontin (OPN) is a pleiotropic glycoprotein expressed in various cell types in animals and in humans, including bone, immune, smooth muscle, epithelial and endothelial cells. Moreover, OPN is found in kidneys (in the thick ascending limbs of the loop of Henle and in distal nephrons) and urine (PMID:30456594).

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