Anticorps Monoclonal anti-P glycoprotein

P glycoprotein Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G6C2

N° de cat : 67258-2-PBS

Synonymes

ABCB1, 1G6C2, EC:7.6.2.2, EC:7.6.2.1, ATP-dependent translocase ABCB1

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Galerie de données de validation

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  WB analysis of HeLa using 67258-2-Ig (same clone as 67258-2-PBS)

  unboiled HeLa cells were subjected to SDS PAGE followed by western blot with 67258-2-Ig (ABCB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67258-2-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 67258-2-Ig (same clone as 67258-2-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67258-2-Ig (ABCB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67258-2-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67258-2-Ig (same clone as 67258-2-PBS)

  unboiled HepG2 cells were subjected to SDS PAGE followed by western blot with 67258-2-Ig (ABCB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67258-2-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67258-2-Ig (same clone as 67258-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67258-2-Ig (ABCB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67258-2-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67258-2-Ig (same clone as 67258-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67258-2-Ig (ABCB1 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67258-2-PBS in a different storage buffer formulation.

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  IF Staining of COLO 320 using 67258-2-Ig (same clone as 67258-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using P glycoprotein antibody (67258-2-Ig, Clone: 1G6C2 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67258-2-PBS in a different storage buffer formulation.

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Informations sur le produit

67258-2-PBS cible P glycoprotein dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: P glycoprotein Protéine recombinante Ag17811
• Nom complet: ATP-binding cassette, sub-family B (MDR/TAP), member 1
• Masse moléculaire calculée: 1280 aa, 142 kDa
• Poids moléculaire observé: 130-150 kDa
• Numéro d’acquisition GenBank: BC130424
• Symbole du gène: P glycoprotein
• Identification du gène (NCBI): 5243
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ABCB1 (also known as P-gp/ P-glycoprotein, MDR1/multidrug-resistance 1) is a plasma membrane protein first discovered in multidrug resistant tumor cells. ABCB1 can extrude a large number of medically relevant compounds from cells and causes drug resistance. ABCB1 is expressed in a variety of human cancers as well as normal tissues including brain, and placenta. Overexpression of ABCB1 correlates with a negative prognosis in several types of cancer. For optimal WB detection, we recommend to avoid boiling the sample after lysis.

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