Anticorps Polyclonal de lapin anti-P16-INK4A

• Phare
• Validé par KD/KO

P16-INK4A Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, FC, Cell treatment, ELISA

Conjugaison

Non conjugué

Publications

302

Avis (4)

N° de cat : 10883-1-AP

Synonymes

ARF, ARF,CDKN2A, CDK4 inhibitor p16 INK4, CDK4 inhibitor p16INK4, CDK4I, CDKN2, CDKN2A, CMM2, INK4, INK4a, MLM, MTS 1, MTS1, Multiple tumor suppressor 1, P16, p16 INK4, p16 INK4a, p16INK4, p16INK4a, P16-INK4A, TP16

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Galerie de données de validation

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  WB analysis using 10883-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10883-1-AP (P16-INK4A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10883-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10883-1-AP (P16-INK4A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK293 cells using 10883-1-AP

  WB result of p16-INK4A antibody (10883-1-AP, 1:2,000) with si-Control and si-p16 transfected HEK-293 cells.

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  IP experiment of HEK-293 using 10883-1-AP

  IP result of anti-P16-INK4A(IP:10883-1-AP, 4ug; Detection:10883-1-AP 1:4000) with HEK-293 cells lysate 1400 ug.

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  IHC staining of human cervical cancer using 10883-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10883-1-AP (P16-INK4A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 10883-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10883-1-AP (P16-INK4A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 10883-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human P16-INK4A (10883-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of HeLa using 10883-1-AP

  1X10^6 HeLa cells were stained with 0.2ug P16-INK4A antibody (10883-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HEK293, cellules HeLa, cellules HepG2, cellules PC-3
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 246 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

10883-1-AP cible P16-INK4A dans les applications de WB, IP, IHC, FC, Cell treatment, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: canin, Humain, singe
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: P16-INK4A Protéine recombinante Ag1328
• Nom complet: cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 16-18 kDa
• Numéro d’acquisition GenBank: BC021998
• Symbole du gène: CDKN2A
• Identification du gène (NCBI): 1029
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Background

p16 is an important cell cycle regulator and acts as a tumor suppressor. It may also be referred to as one of a number of synonyms, including p16INK4a and cyclin-dependent kinase inhibitor 2A.

 What is the molecular weight of P16?

16kDa. P16 is encoded by the CDKN2A gene in humans and is a chain comprising 148 amino acids.

What is the function of p16?

P16 inhibits cells from progressing from G1 into S phase, binding to cyclin-dependent kinase 4 (CDK4) and inhibiting its kinase ability, so that it cannot phosphorylate the retinoblastoma tumor suppressor (RB). Without this phosphorylation, RB does not activate downstream genes, so the G1/S checkpoint cannot be passed and the cell does not proliferate (PMID: 8259215).

 What is the role of p16 in senescence?

In senescence, cells are irreversibly arrested in the cell cycle. P16 is expressed more highly in aging tissue, is associated with intrinsic cellular aging signals such as telomere shortening, and can therefore be used as a marker of senescence (PMID: 9244355; PMID: 19535234). Due to its role in cell cycle arrest, p16 drives the initiation and maintenance of a cellular senescent phenotype.

 What is the role of p16 in cancer?

As a negative regulator of proliferation, p16 is a known tumor suppressor. Mutations in the CDKN2A gene that lead to inactivation of p16 protein have been associated with an increased risk of cancer and are often observed in primary tumors and in cancer cell lines (PMID: 9508208). The inactivation of p16 has been shown to be a key early stage of tumor progression. In a small number of tumor types that are caused by the human papilloma virus (HPV), p16 is in fact overexpressed when RB is inactivated, releasing p16 and causing an accumulation (PMID: 21297668).

Publications

Species	Application	Title
		Nature; Senolytic CAR T cells reverse senescence-associated pathologies.; Authors - Corina Amor
human	WB	Science; PI(3,4)P2-mediated cytokinetic abscission prevents early senescence and cataract formation.; Authors - Federico Gulluni
human		Nat Genet; Early TP53 alterations engage environmental exposures to promote gastric premalignancy in an integrative mouse model.; Authors - Nilay S Sethi
	WB	Cancer Cell; mTORC1 Activation Blocks Braf(V600E)-Induced Growth Arrest but Is Insufficient for Melanoma Formation.; Authors - William Damsky
human	WB	Circulation; Vascular Smooth Muscle Cell Senescence Promotes Atherosclerosis and Features of Plaque Vulnerability.; Authors - Julie Wang
	WB	Cell Metab; SREBP1c-PARP1 axis tunes anti-senescence activity of adipocytes and ameliorates metabolic imbalance in obesity.; Authors - Gung Lee

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Susanne (Verified Customer) (10-11-2022)

blocking: ROTIblock Incubation over night 4°C

• Applications: Western Blot
• Primary Antibody Dilution: 1 : 1000
• Cell Tissue Type: mouse skin tissue

FH

Malak (Verified Customer) (04-22-2021)

Unfortunately, with this antibody, I didn't get a bad at 16 KD, however it was higher (around 20 KD). The suppliers were so professional and offered their help to optimize this antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Huh7 cells extract

FH

Joshua (Verified Customer) (03-12-2020)

MDCKs fixed in 4% paraformaldehyde and stained overnight at 4 C. Excellent, bright stain.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: MDCK

P16-INK4A Antibody Immunofluorescence, validation (1:500 dilution) in MDCK (Cat no:10883-1-AP)

FH

Kyle (Verified Customer) (02-28-2019)

Clean bands, low background

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: small cell lung cancer

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