Anticorps Monoclonal anti-CDKN2A/P16-INK4A

CDKN2A/P16-INK4A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

1C9D3

Avis (1)

N° de cat : 60626-1-Ig

Synonymes

p16, p16 INK4, P16 INK4A, p16-INK4, p16INK4A

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Galerie de données de validation

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  WB analysis using 60626-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60626-1-Ig (P16-INK4A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 60626-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60626-1-Ig (P16-INK4A antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 60626-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using P16-INK4A antibody (60626-1-Ig, Clone: 1C9D3 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Applications testées

• Résultats positifs en WB: SiHa cells, cellules HEK-293, cellules HeLa, cellules TF-1
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

60626-1-Ig cible CDKN2A/P16-INK4A dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CDKN2A/P16-INK4A Protéine recombinante Ag1328
• Nom complet: cyclin-dependent kinase inhibitor 2A
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC021998
• Symbole du gène: CDKN2A
• Identification du gène (NCBI): 1029
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

P16-INK4A is also named as CDKN2A, MLM, Tumor suppressor ARF, Alternative reading frame. The tumor suppressor protein p16Ink4a (encoded from the CDKN2A locus) is often transcriptionally activated in cells undergoing senescence and is one of the main regulators of this program, and it is upregulated in multiple tissues during aging (PMID:17055429). p16-Ink4a is the principal member of the Ink4 family of CDK inhibitors. p16-Ink4a contributes to the regulation of cell cycle progression by inhibiting the S phase. p16Ink4a binds to CDK4/6, inhibiting cyclin D-CDK4/6 complex formation and CDK4/6-mediated phosphorylation of Rb family members. Expression of p16-Ink4a maintains the Rb family members in a hypophosphorylated state, which promotes binding to E2F1 and leads to G1 cell cycle arrest (PMID: 21297668).

Publications

Species	Application	Title
human,mouse	WB	Nat Aging; Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer; Authors - Yifei Cheng
mouse	WB	Hum Vaccin Immunother; Nanovaccine loaded with seno-antigen target senescent cells to improve metabolic disorders of adipose tissue and cardiac dysfunction; Authors - Kexin Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marisa (Verified Customer) (07-31-2025)

We used the p16 antibody from Proteintech for immunofluorescence on mouse brain, followed by confocal microscopy. The antibody produced a strong, specific signal and Minimal background and high signal-to-noise ratio made it easy to interpret.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/250
• Cell Tissue Type: Brain

CDKN2A/P16-INK4A Antibody Immunofluorescence validation (1/250 dilution) in Brain (Cat no:60626-1-Ig)

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