Anticorps Monoclonal anti-CDKN2A/P16-INK4A

CDKN2A/P16-INK4A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C9D3

N° de cat : 60626-1-PBS

Synonymes

p16, p16 INK4, P16 INK4A, p16-INK4, p16INK4A

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Galerie de données de validation

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  WB analysis using 60626-1-Ig (same clone as 60626-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60626-1-Ig (P16-INK4A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60626-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60626-1-Ig (same clone as 60626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60626-1-Ig (P16-INK4A antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60626-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60626-1-Ig (same clone as 60626-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using P16-INK4A antibody (60626-1-Ig, Clone: 1C9D3 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60626-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60626-1-PBS cible CDKN2A/P16-INK4A dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CDKN2A/P16-INK4A Protéine recombinante Ag1328
• Nom complet: cyclin-dependent kinase inhibitor 2A
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC021998
• Symbole du gène: CDKN2A
• Identification du gène (NCBI): 1029
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

P16-INK4A is also named as CDKN2A, MLM, Tumor suppressor ARF, Alternative reading frame. The tumor suppressor protein p16Ink4a (encoded from the CDKN2A locus) is often transcriptionally activated in cells undergoing senescence and is one of the main regulators of this program, and it is upregulated in multiple tissues during aging (PMID:17055429). p16-Ink4a is the principal member of the Ink4 family of CDK inhibitors. p16-Ink4a contributes to the regulation of cell cycle progression by inhibiting the S phase. p16Ink4a binds to CDK4/6, inhibiting cyclin D-CDK4/6 complex formation and CDK4/6-mediated phosphorylation of Rb family members. Expression of p16-Ink4a maintains the Rb family members in a hypophosphorylated state, which promotes binding to E2F1 and leads to G1 cell cycle arrest (PMID: 21297668).

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