Anticorps Recombinant de lapin anti-P21

P21 Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3N14

N° de cat : 82669-2-PBS

Synonymes

CDKN1A, P21;CDKN1A, 3N14, CDK-interacting protein 1, CIP1

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Galerie de données de validation

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  WB analysis using 82669-2-RR (same clone as 82669-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82669-2-RR (P21 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82669-2-PBS in a different storage buffer formulation.

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  WB analysis using 82669-2-RR (same clone as 82669-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82669-2-RR (P21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82669-2-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 82669-2-RR (same clone as 82669-2-PBS)

  IP result of anti-P21 (IP:82669-2-RR, 4ug; Detection:82669-2-RR 1:800) with MCF-7 cells lysate 760 ug. This data was developed using the same antibody clone with 82669-2-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 82669-2-RR (same clone as 82669-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using P21 antibody (82669-2-RR, Clone: 3N14 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82669-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82669-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82669-2-RR against Human P21 were performed. The affinity constant is 112 nM.

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Informations sur le produit

82669-2-PBS cible P21 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: P21 Protéine recombinante Ag0368
• Nom complet: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC000275
• Symbole du gène: p21
• Identification du gène (NCBI): 1026
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

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