Anticorps Monoclonal anti-P2RX4

P2RX4 Monoclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1A5A6

N° de cat : 66416-1-Ig

Synonymes

ATP receptor, P2RX4, P2X purinoceptor 4, P2X4, P2X4R, Purinergic receptor

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Galerie de données de validation

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  WB analysis using 66416-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66416-1-Ig (P2RX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66416-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66416-1-Ig (P2RX4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 66416-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66416-1-Ig (P2RX4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66416-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66416-1-Ig (P2RX4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 66416-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66416-1-Ig (P2RX4 Antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human kidney using 66416-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66416-1-Ig (P2RX4 Antibody) at dilution of 1:400 (under 40x lens).

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  FC experiment of HeLa using 66416-1-Ig

  1X10^6 HeLa cells were stained with 0.20 ug Anti-Human P2RX4 (66416-1-Ig, Clone:1A5A6) (red) or 0.20 ug isotype control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules Neuro-2a, tissu cérébral de lapin, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66416-1-Ig cible P2RX4 dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: P2RX4 Protéine recombinante Ag4445
• Nom complet: purinergic receptor P2X, ligand-gated ion channel, 4
• Masse moléculaire calculée: 388 aa, 43 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC033826
• Symbole du gène: P2RX4
• Identification du gène (NCBI): 5025
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs). P2RX4 (P2X purinoceptor 4) is a receptor for ATP acting as a ligand gated ion channel. The calculated molecular weight of P2RX4 is 43 kDa, larger apparent molecular weights of 50-80 kDa have been reported, possibly due to post-translational glycosylation (PMID: 24040145, 15262999; 29382907; 12566439).

Publications

Species	Application	Title
mouse	WB	Clin Transl Med; P2X4 receptor modulates gut inflammation and favours microbial homeostasis in colitis; Authors - Peijie Zhong

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