Anticorps Monoclonal anti-P2RX4

P2RX4 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A5A6

N° de cat : 66416-1-PBS

Synonymes

ATP receptor, P2RX4, P2X purinoceptor 4, P2X4, P2X4R, Purinergic receptor

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Galerie de données de validation

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  WB analysis using 66416-1-Ig (same clone as 66416-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66416-1-Ig (P2RX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66416-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66416-1-Ig (same clone as 66416-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66416-1-Ig (P2RX4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66416-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66416-1-Ig (same clone as 66416-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66416-1-Ig (P2RX4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66416-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66416-1-Ig (same clone as 66416-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66416-1-Ig (P2RX4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66416-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66416-1-Ig (same clone as 66416-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66416-1-Ig (P2RX4 Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66416-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66416-1-Ig (same clone as 66416-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66416-1-Ig (P2RX4 Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66416-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66416-1-PBS cible P2RX4 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: P2RX4 Protéine recombinante Ag4445
• Nom complet: purinergic receptor P2X, ligand-gated ion channel, 4
• Masse moléculaire calculée: 388 aa, 43 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC033826
• Symbole du gène: P2RX4
• Identification du gène (NCBI): 5025
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs). P2RX4 (P2X purinoceptor 4) is a receptor for ATP acting as a ligand gated ion channel. The calculated molecular weight of P2RX4 is 43 kDa, larger apparent molecular weights of 50-80 kDa have been reported, possibly due to post-translational glycosylation (PMID: 24040145, 15262999; 29382907; 12566439).

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